摘要
为快速、准确地分析烟草香味成分,基于保留指数(RI)建立了241种烟草香味成分的GC-MS/MS分析方法。通过检测标准品确定241种香味成分的RI,同时利用RI准确区分标准品中的异构体,并利用RI实现多靶标GC-MS/MS法采集时间窗口的快速调整。结果表明:①测定的241种香味成分的RI中有212种的RI与NIST17库中收录数值的差值<10,另25种还未被NIST17收录;②利用RI可以明显提高异构体之间定性分析的准确性和可靠性;③利用RI对目标物在不同色谱条件下的保留时间进行预测,232种目标物的时间偏差<0.05 min,9种目标物的时间偏差在0.05~0.10 min以内。表明RI在复杂烟草基质中的稳定性良好,可快速准确地预测多靶标GC-MS/MS法中的采集时间窗口。
In order to qualitatively analyze tobacco aroma components accurately and rapidly,a gas chromatography-tandem mass spectrometry(GC-MS/MS)method was established based on the retention indices(RI)of 241 tobacco aroma components.The retention indices of 241 target components were determined first by using standard samples,which accurately differentiated isomers in those standard samples and to realize rapid adjustment of the acquisition time window of the multi-analyte GC-MS/MS analytical method.The results showed that:1)Among the retention indices of 241 aroma components,the differences between experimental determined values and those from the NIST17 database were less than 10 for 212 of the components,and the retention indices of the other 25 components had not been recorded in NIST17 database.2)Adding retention index successfully promoted the accuracy and reliability of qualitative analysis among isomers.3)Retention indices of the targets were used to predict the retention times of the target compounds under different chromatographic conditions,the time deviations of 232 components were within 0.05 min,and that of the other 9 components were within 0.05-0.10 min.It proved that the retention index had good stability in complex tobacco matrix and could be used to accurately predict the acquisition time window in multi-analyte GC-MS/MS method rapidly.
作者
任宗灿
陈黎
史天彩
王晓瑜
崔华鹏
谢复炜
刘惠民
REN Zongcan;CHEN Li;SHI Tiancai;WANG Xiaoyu;CUI Huapeng;XIE Fuwei;LIU Huimin(Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,China)
出处
《烟草科技》
EI
CAS
CSCD
北大核心
2020年第8期24-35,共12页
Tobacco Science & Technology
