摘要
Phylogenomic approaches are growingly applied in the systematic studies of animals with the rapid advancement of sequencing and analytical tools.Aiming to accelerate the systematic studies using genome-scale data in China,this paper outlines the commonly used techniques for generating phylogenomic datasets:RNA-seq based transcriptome,targetenrichment based UCEs and AHE,RAD-seq,whole genome sequencing,and briefly discusses the pros and cons of each technique.Major analytical procedures for the 1 KITE transcriptome pipeline,the PHYLUCE(UCEs)and AHE pipelines and the PLWS(low-coverage whole genome sequencing)pipeline are summarized,and recent achievements valuable for phylogenomic analyses are introduced.
基金
supported by the Advanced Talents Incubation Program of the Hebei University to Junxia Zhang(521000981324)。