摘要
为探究不同年限连续栽培百合的设施土壤细菌和真菌群落组成变化及其与环境因子间的关系,采用Mi Seq高通量测序技术,对连作4年(C4)、连作5年(C5)和连作7年(C7)设施百合红壤中细菌和真菌群落组成和多样性的变化进行了研究。结果显示,所有样品测序后获得细菌和真菌有效序列分别是249003和451044个,细菌和真菌OTUs总数分别是906和298个。Alpha多样性分析显示,随连作年限延长设施百合红壤中细菌香农指数和辛普森指数显著升高,真菌香农指数和辛普森指数显著下降。在物种组成分析中,属于细菌优势菌种的有变形菌门(Proteobacteria)、放线菌门(Actinomycetes)、酸杆菌门(Acidobacteria)、绿弯菌门(Chloroflexi),且C4、C5和C7处理土壤中优势菌种占细菌总数比例分别是85.17%、81.04%和81.64%;而真菌菌门中,只有子囊菌门(Ascomycota)一个优势菌种,并且C4、C5和C7处理土壤中优势菌种占真菌总数比例分别93.69%、92.20%、84.31%。不同连作年限土壤中,子囊菌门镰刀菌属(Fusarium)丰度比例达44.02%~58.83%。相关性分析显示,p H值、有机碳、总氮与变形菌门、放线菌门、酸杆菌门丰度显著相关;p H与青霉属(Penicillium)显著正相关,与镰刀菌属显著负相关。由此可以认为,连作后土壤细菌微生物群落多样性指数显著上升,真菌微生物多样性显著下降,同时土壤中细菌的优势菌种有变形菌门、放线菌门、酸杆菌门、绿弯菌门,在真菌群落组成中子囊菌门占主导,连作后子囊菌门中占主导地位的镰刀菌属数量的增长可能是引起设施百合红壤连作障碍的主要原因之一。
The purpose of this study was to investigate the changes of bacterial and fungal community compositions and their relationship with environmental factors in Red Soil under different continuous cropping yearsof lily.The composition and diversity of soil bacterial and fungal communities under continuous cropping of lily for 4 years(C4),5 years(C5)and 7 years(C7)were analyzed by using Mi Seq high-throughput sequencing method.The results showed that the effective bacterial and fungal sequences obtained after sequencing of all samples were 249003 and 451044,respectively,and the total numbers of bacterial and fungal OTUs were 906 and 298.Alpha diversity analysis showed that the Shannon index and Simpsoneven index of bacteriawere significantly increasedand those of fungi were significantly decreased with the continuous cropping years of lily undergreenhouse cultivation.The dominant bacterial species were Proteobacteria,Actinomycetes,Acidobacteria,and Chlorobacteria.And the dominant bacterial species in C4,C5,and C7 accounted for 85.17%,81.04%and 81.64%of the total bacterial species,respectively.Among the fungi,only Ascomycota was the dominant species,and the dominant species in C4,C5 and C7 accounted for 93.69%,92.20%and 84.31%of the total fungi species,respectively.The abundances of Fusarium ascomycetes were 44.02%~58.83%in the three soils.Correlation analysis showed that p H value,organic carbon and total nitrogen were significantly correlated with the abundances of Proteobacteria,Aactinomycetes andAcidobacteria.The p H value was positively correlated with Penicilliumand negatively correlated with Fusarium.The results of this study indicated that the diversity index of soil bacterial microbial community increased significantly,and the diversities of fungal microbial community decreased significantly after continuous cropping.Meanwhile,the dominant bacterial species in soils were Proteobacteria,Actinomycetes,Acidobacteria,and Chloromonas,and Ascomycota dominated in the fungal community.The increase of Fusarium number d
作者
李晶晶
续勇波
LI Jing-jing;XU Yong-bo(College of Resources and Environment,Yunnan Agricultural University,Kunming 650201,China;College of Tobacco Science,Yunnan Agricultural University,Kunming 650201,China)
出处
《土壤通报》
CAS
CSCD
北大核心
2020年第2期343-351,共9页
Chinese Journal of Soil Science
基金
国家自然科学基金(31660601,31260503)资助。
关键词
连作年限
高通量测序技术
优势菌种
群落组成
Continuous cropping years
High-throughput sequencing technology
Dominant species
Community composition
