摘要
酰胺醇类抗生素氟苯尼考具有抗菌谱广、用量低、细菌耐药性低及毒性小等优点,已被广泛应用于兽医实践。采用对硝基甲苯为内标,以单峰甲基信号为定量信号峰,建立了定量分析氟苯尼考和对乙酰氨基苯酚的核磁共振氢谱定量方法。该方法方便快捷,适用于氟苯尼考的质量控制分析。通过本实验使学生对于核磁共振波谱有了更深的认识,培养了学生学以致用、理论联系实际和解决实际问题的能力。
Fluphenicol, an amido alcohol antibiotic, has been widely used in veterinary practice due to its advantages of broad antibacterial spectrum, low dosage, low bacterial resistance and low toxicity. Quantitive proton nuclear magnetic resonance method for quantitative analysis of fluorbenicol and acetaminophenol was established by using paranitrotoluene as internal standard and single peak methyl signal as quantitative signal peak. The method was convenient and suitable for the quality control analysis of fluphenicol. Through this experiment, the students had a deeper understanding of the NMR spectrum, and cultivated the students’ ability to apply what they have learned, combine theory with practice and solve practical problems.
作者
毛秋平
梁向晖
MAO Qiu-Ping;LIANG Xiang-Hui(Key Laboratory of Functional Molecular Engineering of Guangdong Province,School of Chemistry and Chemical Engineering,South China University of Technology,Guangzhou 510640,China)
出处
《化学教育（中英文）》
CAS
北大核心
2020年第14期47-52,共6页
Chinese Journal of Chemical Education
基金
华南理工大学探索性实验项目(Y9180680)。
关键词
氟苯尼考
核磁共振氢谱定量
综合化学实验
对乙酰氨基苯酚
fluorfenicol
quantitive proton nuclear magnetic resonance spectroscopy
comprehensive chemical experiment
acetaminophenol
