摘要
对采自祁连山地区的一株野生食用菌进行分离,通过形态学及其ITS序列的分子系统发育分析鉴定为絮缘蘑菇Agaricus subfloccosus。以分离纯化获得的菌株作为实验材料,对该种的生物学特性和驯化栽培进行研究。检测了不同碳源、氮源、温度、pH、无机盐、维生素在固体培养条件下对絮缘蘑菇菌丝生长的影响,对以上6个因素进行单因素试验,从中挑选出3个较优因素的最优水平进行正交试验。结果表明:实验范围内,絮缘蘑菇菌丝生长最佳碳源为蔗糖,最佳氮源为硝酸钾,最佳无机盐为硫酸镁,最佳维生素为VB12,最佳温度为16℃,最佳pH为6。影响较大的3个因素为碳源、无机盐和pH,絮缘蘑菇最适的营养因子最佳组合配方为:蔗糖25g/L,硝酸钾2g/L,硫酸镁2g/L,pH为5.0。驯化栽培过程中,栽培配方为:木屑79%、高粱籽5%、玉米面5%、石膏1%、麸皮10%。温度16℃使絮缘蘑菇菌丝在90d左右满袋,覆土可培育出子实体。
A fungal isolate from Qilian Mountain was identified as Agaricus subfloccosus based on morphological characteristics and molecular phylogenetic analysis of ITS sequence.The biological characteristics and domestic cultivation of the fungus were studied.The effects of carbon source,nitrogen source,temperature,pH value,inorganic salt and vitamins on growth of the mycelium were investigated by single factor test and optimized by orthogonal experiments.The results showed that carbon source,inorganic salt and pH were considered to be important to mycelial growth.The mushroom grew well on PDA medium(25 g sucrose,2 g potassium nitrate,2 g magnesium sulfate and 1000 mL water)with additional VB12.The optimal pH was 5.0.For the sack cultivation,79%sawdust,5%bran,5%corn starch,1%gypsum and 10%wheat bran were used,and the mycelia were sackful in 90 days at 16°C.Fruiting occurred after casing.
作者
梁倩倩
宋利茹
牛鑫
席亚丽
冯九海
曹选莉
贺宇杉
祝建烜
魏生龙
LIANG Qian-Qian;SONG Li-Ru;NIU Xin;XI Ya-Li;FENG Jiu-Hai;CAO Xuan-Li;HE Yu-Shan;ZHU Jian-Xuan;WEI Sheng-Long(Gansu Engineering Laboratory of Applied Mycology,Hexi University,Zhangye,Gansu 734000,China;Qilianshan Collaborative Innovation Center of Edible Mushroom Industry,Hexi University,Zhangye,Gansu 734000,China;Gansu Key Laboratory of Genetics and Breeding of Edible Mushroom Industry,Hexi University,Zhangye,Gansu 734000,China;College of Life Sciences and Engineering,Hexi University,Zhangye,Gansu 734000,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2020年第7期1301-1311,共11页
Mycosystema
基金
国家自然科学基金地区基金(31860582,31660590,31860709)
高校协同创新团队项目(2016C-02)
甘肃省自然科学基金(18JR3RG207)
甘肃省科技计划小巨人企业培育计划项目(17CX1JG091)
甘肃省引导科技创新发展专项资金(2017zx-10)。
关键词
絮缘蘑菇
生物学特性
正交试验
驯化栽培
Agaricus subfloccosus
biological characteristics
orthogonal experiments
domestication
