摘要
抑郁是一种以情绪低落、快感缺失为主要症状的精神类疾病,严重危害人类身心健康。合适的抑郁动物模型,对于抑郁的研究和抗抑郁新药研发等具有重要意义,而实验动物、造模方法、评价指标的合理选择与匹配,是评价其科学性和有效性的关键。通过系统查阅PubMed、中国知网(CNKI)等数据库中的抑郁研究文献,该文从实验动物选择、造模原理和方法、特点、评价指标及其在中医药中的应用等方面,总结了近年来常用的抑郁动物模型的研究进展。常用的抑郁动物模型造模方法有应激法、糖皮质激素诱导法、利血平诱导法、脂多糖诱导法、手术造模法、基因敲除法及联合应用造模法等。应激法造模可较好地模拟临床抑郁患者的症状,但存在建模时间长、耗资较大等不足;糖皮质激素法、利血平法、脂多糖法诱导的抑郁动物模型,建模时间短、可控性较好,但可靠性较差;手术造模的致病机制与临床抑郁患者的发病机制吻合度较高,但存在术后恢复期较长的缺陷;基因敲除动物模型可用于研究特定基因在抑郁中的特定作用,但其在抑郁研究中的适用性可能受到限制;联合应用造模法提高了抑郁动物模型的可靠性及数据的准确性,得到越来越多的重视。该文可为抑郁的病理机制、抗抑郁药物的筛选及评价等研究中动物模型的选择提供参考。
Depression is a kind of mental disease with main symptoms of low mood and lack of pleasure,which seriously endangers human health.An appropriate depressive animal model is of great significance for the study of depression and new antidepressant drugs,while the suitable selection and matching of experimental animals,modeling methods and evaluation indexes are critical to eva-luate the scientificity and effectiveness of the depressive animal model.The study advance of depressive animal models in the aspects of experimental animal selection,modeling principle and method,characteristics,evaluation indexes and their application in traditional Chinese medicine are summarized through the systematic review of relevant literatures in PubMed,CNKI and other databases.The depressive animal modeling methods utilized in recent studies include stress,glucocorticoid induction,reserpine induction,lipopolysaccharide induction,surgical modeling,gene knockout,joint application modeling methods.Stress method is better to simulate the depressive symptoms of clinical patients,whereas there are some deficiencies,such as long modeling time and large cost.The depressive animal models induced by glucocorticoid,reserpine and lipopolysaccharide have the advantages of short modeling time and good controllability,but with a poor reliability.The pathogenesis of surgical modeling is highly matched with that of clinical depressive patients,whereas it has the defect of long postoperative recovery period.Gene knockout models can be used to study the precise role of specific genes in depression.However,its applicability may be restricted in studies on depression.The joint application modeling method can improve its reliability and accuracy,and attracts more and more attention.This paper provides a reference for the selection of animal models in future studies of pathological mechanism of depression,and screening and evaluation of antidepressant drugs.
作者
王雪雪
陶柱萍
厉颖
李灿委
范孟然
刘卫红
韦桂宁
高鹏飞
WANG Xue-xue;TAO Zhu-ping;LI Ying;LI Can-wei;FAN Meng-ran;LIU Wei-hong;WEI Gui-ning;GAO Peng-fei(College of Pharmacy and Chemistry,Dali University,Dali 671000,China;College of Public Health,Dali University,Dali 671000,China;Department of Agriculture and Biological Science,Dali University,Dali 671000,China;Department of Pharmacology,Guangxi Institute of Chinese Medicine and Pharmaceutical Science,Nanning 530022,China;National Joint Engineering Research Center for the Development of Medicinal Special Insects,Dali 671000,China;Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D,Dali University,Dali 671000,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2020年第11期2473-2480,共8页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81260676,81560663,31760158,81960712)
云南省教育厅科学研究基金重点项目(2013Z154)
云南省地方本科高校(部分)基础研究联合专项项目(2018FH001-097)。
关键词
抑郁
动物模型
中医药
应用进展
depression
animal model
traditional Chinese medicine
application progress
