摘要
为了探讨拟南芥O-岩藻糖基转移酶(SPINDLY)在病原体相关分子模式诱导抗性中的作用,该研究以SPINDLY缺失拟南芥突变体spy-3为实验材料,从叶片表型、病情指数、病菌定殖量以及丁香假单胞菌(Pst DC3000)关键基因的表达水平等指标,系统考察了SPINDLY在壳寡糖诱导拟南芥抗Pst DC3000中的功能。结果显示:(1)spy-3突变体比野生型更易被Pst DC3000侵染。(2)与病菌侵染组相比,壳寡糖预处理明显缓解植株叶片黄化现象,显著降低Pst DC3000的定殖量。(3)壳寡糖预处理的spy-3植株中水杨酸和茉莉酸途径相关基因的表达量及水杨酸和茉莉酸含量均较病菌侵染组明显升高。(4)壳寡糖在spy-3中的诱抗效果与野生型相比无明显差别。研究表明,SPINDLY在植物先天免疫过程发挥重要作用,但并不影响壳寡糖的诱导抗性。
In this study,the chitosan oligosaccharide(COS)induced resistance to Pst DC3000 was investigated in SPINDLY deficient Arabidopsis mutant spy-3.From multilevel indices,including phenotype data,the disease index,the bacterial numbers in infected leaves and the expression of Pst DC3000 marker genes.The function of SPINDLY in COS induced resistance was systematically investigated.The results showed that:(1)spy-3 mutant was more susceptible to Pst DC3000 infection than wild type.(2)Compared with the Mock+Pst group,COS pretreatment significantly relieved the leaf symptoms,inhibited the proliferation of Pst DC3000,and reduced the disease index.(3)SA and JA-mediated pathway related genes and the contents of SA and JA were up-regulated significantly in COS pre-treated spy-3 plants.(4)The effect of COS induced resistance in spy-3 has no significant difference compared with wild type.The results suggested the essential role of SPY in plant innate immunity.However,COS still effectively induce the disease resistance in spy-3.
作者
刘俊杰
贾晓晨
赵小明
王文霞
尹恒
LIU Junjie;JIA Xiaochen;ZHAO Xiaoming;WANG Wenxia;YIN Heng(Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116023, China;University of Chinese Academy of Sciences, Beijing 100049, China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2020年第5期766-772,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家重点研发计划(2017YFD0200900,2017YFD0200902)
国家自然科学基金(31971217)
DICP(I201905)
辽宁振兴人才计划(XLYC1807041)。
