摘要
目的:探讨SDF-1/CXCR4介导通路对小鼠骨折愈合的影响。方法:40只C57BL雄性小鼠随机分为抑制组与对照组,采用切开暴露股骨中段制造股骨干横行骨折,插入髓内针制作小鼠骨折愈合模型,抑制组小鼠每次每只每12小时1.25mg/kg局部皮下注射CXCR4抑制剂AMD3100,对照组小鼠注射等量磷酸缓冲盐溶液;在模型构建后第1、3、5、8周处死小鼠,采用放射学X线片观测其影像学改变;采用免疫组织化学观测指标SDF-1、ERK、AKT在组织中的表达水平;采用实时定量荧光PCR观测指标COL1、COL2、CXCR4、VEGF在组织中的表达水平。结果:小鼠单侧开放性股骨干横形骨折+髓内针固定模型构建成功,适用于小鼠骨折愈合的研究。两组COL1、CXCR4、VEGF在组织中表达均逐渐上升,至第3周时达到高峰,后逐渐下降,且同时间对照组均高于抑制组,差异均有统计学意义(P<0.05);两组COL2均在第1周即达到高峰,后快速下降,且同时间对照组均高于抑制组,差异均有统计学意义(P<0.05)。两组SDF-1、ERK、AKT在组织中表达均逐渐上升,至第3周时达到高峰,后逐渐下降,且同时间对照组均高于抑制组,差异均有统计学意义(P<0.05)。结论:在小鼠中抑制SDF-1/CXCR4通路将会延缓骨折愈合。
Objective:To investigate the the effect of SDF-1/CXCR4 pathway on fracture healing in mice.Method:A total of 40 C57BL male mice were randomly divided into the inhibition group and the control group.All mice operated by exposing the middle part of femur and making transverse fracture of femoral shaft,inserting intramedullary needle to make model of fracture healing in mice.The inhibition group was injected CXCR4 inhibitor AMD31001.25 mg/kg every 12 hours at a time by partial subcutaneous.The control group was injected with the same amount of phosphate buffer solution.The mice were sacrificed at week 1,3,5 and 8 after model construction,and radiological X-ray was used to observe the imaging changes.The expression levels of SDF-1,ERK and AKT in tissues were used as immunohistochemical observation indexes.Real-time quantitative fluorescence PCR was used to observe the expression levels of COL1,COL2,CXCR4 and VEGF in tissues.Result:The model of fracture healing in mice was established successfully by unilateral open transverse fracture of femoral shaft+intramedullary needle fixation.The expressions of COL1,CXCR4 and VEGF in the tissues of the two groups increased gradually,reached the peak at week 3,and then decreased gradually.In the same period,the control group was higher than the inhibition group,the differences were statistically significant(P<0.05).The COL2 in both groups reached its peak in the first week,and then decreased rapidly.In the same period,the COL2 in the control group was higher than that in the inhibition group,the differences were statistically significant(P<0.05).The expressions of SDF-1,ERK and AKT in the tissues of the two groups increased gradually,reached the peak at week 3,and then decreased gradually.Moreover,the expression of SDF-1,ERK and AKT in the control group were higher than those in the inhibition group at that time,the differences were statistically significant(P<0.05).Conclusion:Inhibition of SDF-1/CXCR4 pathway in mice will delay fracture healing.
作者
唐昊
陈顺有
卢晓坤
陈福明
TANG Hao;CHEN Shunyou;LUXiaokun;CHEN Fuming(Fuzhou Second Hospital Affiliated to Xiamen University,Fuzhou 350007,China;不详)
出处
《中外医学研究》
2020年第19期6-9,共4页
CHINESE AND FOREIGN MEDICAL RESEARCH
基金
福建省卫生计生科研人才培养项目(2017-CX-46)。
