摘要
目的:探讨miR-141-3p对胃癌细胞增殖和迁移及核转录因子κB(NF-κB)信号通路的影响。方法:培养人胃癌细胞株BGC-823,采用脂质体转染技术将miR-141-3p模拟物(miR-141-3p mimics)转染至BGC-823细胞构建miR-141-3p过表达的BGC-823细胞株。实时荧光定量聚合酶链式反应(qRT-PCR)检测转染效果,噻唑蓝比色法检测miR-141-3p对BGC-823细胞增殖的影响,Transwell实验检测miR-141-3p对BGC-823细胞迁移的影响,Western blot法检测NF-κB信号通路相关NF-κB p65、p-IKK-α和p-IKB-α蛋白的表达情况。结果:与对照组和阴性对照组比较,miR-141-3p组BGC-823细胞中miR-141-3p的表达水平为2.39±0.27,高于对照组(1.00±0.09)和阴性对照组(1.01±0.10),差异均有统计学意义(均P<0.05);过表达miR-141-3p后,miR-141-3p组的迁移细胞数为(47.64±5.65)个,低于对照组[(106.22±12.14)个]和阴性对照组[(110.40±12.26)个],差异均有统计学意义(均P<0.05);BGC-823细胞中NF-κB p65、p-IKK-α和p-IKB-α蛋白的表达水平下调(P<0.05)。结论:miR-141-3p可抑制人胃癌BGC-823细胞的增殖和迁移,其作用机制可能与抑制NF-κB信号通路的激活有关。
Objective To investigate the effects of miR-141-3p on proliferation and migration of gastric cancer cells and nuclear factor-κB(NF-κB)signaling pathway.Methods Human gastric cancer cell line BGC-823 was cultured,and miR-141-3p mimetic(miR-141-3p mimics)was transfected into BGC-823 cells by lipofection.The miR-141-3p overexpressed BGC was constructed.Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)was used to detect the transfection effect.The proliferation of BGC-823 cells was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.Transwell assay was used to detect the effect of miR-141-3p on BGC-823 cell migration.The expressions of NF-κB p65,p-IKK-αand p-IKB-αprotein in NF-κB signaling pathway were detected by western blot.Results Compared with the control group and the negative control group,the expression level of miR-141-3p in BGC-823 cells of the miR-141-3p group was(2.39±0.27),which was higher than(1.00±0.09)of the control group and(1.01±0.10)of the negative control group(P<0.05).The number of migrating cells in the miR-141-3p group was(47.64±5.65),which was lower than(106.22±12.14)in the control group and(110.40±12.26)in the negative control group(P<0.05).The expression levels of NF-κB p65,p-IKK-αand p-IKB-αprotein in BGC-823 cells were down-regulated(P<0.05).Conclusion MiR-141-3p can inhibit the proliferation and migration of human gastric cancer BGC-823 cells,which may be related to the inhibition of NF-κB signaling pathway activation.
作者
王飞
杨继鑫
陈智年
Wang Fei;Yang Jixin;Chen Zhinian(The Second Department of General Surgery,Xinxiang Central Hospital,Xinxiang 453000,China;Department of Thyroid and Breast Vascular Surgery,The First Affiliated Hospital of Air Force Military Medical University,Xi’an 710000,China)
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2020年第7期556-559,共4页
Chinese Journal of Oncology
