摘要
[目的]筛选高产漆酶菌株。[方法]利用愈创木酚-PDA平板筛选高产漆酶菌株,单因素实验确定最佳产酶条件。[结果]筛选到一株高产漆酶菌株,编号QMJZ-5。结合形态观察和5.8S rDNA-ITS序列分析,确定该菌株是血红密孔菌(Pycnoporus coccineus),优化发酵培养基:甘油20 g/L,豆粕6.0 g/L,香兰素0.15 g/L,阿魏酸0.15 g/L,KH2 PO41.0 g/L,Na2HPO4·12H2O 0.2 g/L,CuSO4·5H2O 1.5 mmol/L,初始pH 5.0,30℃,发酵8 d,产酶55 U/mL。[结论]筛选到1株高产漆酶菌株。
[Objective]The laccase-producing strain was obtained by separation.[Method]The plate substrate chromogenic of guaiacol was used to sparate laccase-producting strains.Single factor experiment was then used to get optimal culture media and conditions.[Result]A high-yield laccase strain was obtained and named QMJZ-5.On the basis of its 5.8S rDNA-ITS gene-sequencing analysis and morphological observation,the strain was classified as the genus of Pycnoporus coccineus.The optinal medium was:glycerine 20 g/L,soybean meal powder 6.0 g/L,vanillin 0.15 g/L,ferulaic acid 0.15 g/L,KH2PO41.0 g/L,Na2HPO4·12H2O 0.2 g/L,CuSO4·5H2O 1.5 mmol/L,pH 5.0,200 r/min,30℃.Enzymea activity under the optimal conditions was 55 U/mL,which was 15 times of the riginal medium.[Conclusion]A laccase-producing strain was obtained by separation.
作者
孙荣
刘建民
任明
张雷
SUN Rong;LIU Jian-min;REN Ming;ZHANG Lei(Shandong Huishilai Biotechnology Co.,Ltd,Jinan 250100,China)
出处
《生物技术》
CAS
2020年第3期280-284,共5页
Biotechnology
关键词
漆酶
真菌
筛选
发酵条件
优化
laccase
fungi
isolation
fermentation conditions
optimization
