摘要
目的探究米诺地尔联合地塞米松对肾缺血再灌注损伤大鼠肾功能指标和氧化应激指标的影响。方法 40只SD大鼠通过ip 1%戊巴比妥钠溶液4 mg/kg建立肾缺血再灌注损伤模型,分为假手术组、模型组、地塞米松组、米诺地尔组、米诺地尔联合地塞米松组,每组8只。假手术组不进行肾缺血再灌注处理;模型组术前30 min ip生理盐水1 mL;地塞米松组术前30 min ip 4 mg/kg地塞米松磷酸钠注射液;米诺地尔组术前30 min ip 0.6 mg/kg米诺地尔;米诺地尔联合地塞米松组术前30 min ip 0.3 mg/kg米诺地尔和2 mg/kg地塞米松磷酸钠注射液。考察米诺地尔联合地塞米松对肾缺血再灌注损伤大鼠肾功能指标和氧化应激指标的影响。结果地塞米松组、米诺地尔组、米诺地尔联合地塞米松组的血清尿素氮(BUN)、肌酐(Cr)值和肾组织丙二醛(MDA)值均显著低于模型组(P<0.05、0.01),以米诺地尔联合地塞米松组最为显著(P<0.01);肾组织匀浆总超氧化物歧化酶(T-SOD)活性均显著高于模型组(P<0.01),以米诺地尔联合地塞米松组最为显著(P<0.01)。结论地塞米松和米诺地尔预处理均可以显著减轻急性肾缺血再灌注的损伤,且两者对急性肾缺血再灌注损伤的保护作用总体相近,联合用药预处理的保护作用则更加显著。
Objective To investigate the effect of minoxidil combined with dexamethasone on renal function indicators and oxidative stress indexes in rats with kidney ischemic reperfusion injury. Methods 40 SD mice were ip with 1% sodium pentobarbital solution to establish the kidney ischemic reperfusion injury model, and they were divided into sham group, model group, dexamethasone group, minoxidil group, and minoxidil combined with dexamethasone group, with 8 mice in each group. Renal ischemia reperfusion was not performed in the sham group. The model group were ip normal saline 1 mL 30 min before operation. The dexamethasone group were ip 4 mg/kg Dexamethasone Sodium Phosphate Injection 30 min before operation. The minoxidil group were ip 0.6 mg/kg minoxidil 30 min before operation. The minoxidil combined with dexamethasone group were ip 2 mg/kg Dexamethasone Sodium Phosphate Injection and 0.3 mg/kg minoxidil 30 min before operation. Results The BUN, Cr in serum, and MDA value in kidney tissue were significantly lower in the dexamethasone group, minoxidil group, and minoxidil combined with dexamethasone group than those in the model group activity in renal tissue homogenate was significantly higher than that in the model group(P < 0.01), and that in the minoxidil combined with dexamethasone group was the most significant(P < 0.01). Conclusion Both dexamethasone and minoxidil pretreatment can significantly reduce the acute renal ischemia reperfusion injury. The protective effects of the two are similar and the combination of pretreatment with medication is better in results.
作者
刘钰林
陈珮如
梁毅敏
梁天翔
吴镇鹏
林沛豪
罗静彤
薛勤
LIU Yu-lin;CHEN Pei-ru;LIANG Yi-min;LIANG Tian-xiang;WU Zhen-peng;LIN Pei-hao;LUO Jing-tong;XUE Qin(Second Clinical College,Guangzhou Medical University,Guangzhou 511436,China;Function Experimental Center of Basic College,Guangzhou Medical University,Guangzhou 511436,China;Department of Pharmacology,Guangzhou Medical University,Guangzhou 511436,China)
出处
《现代药物与临床》
CAS
2020年第6期1064-1068,共5页
Drugs & Clinic
基金
2019—2020年度院级大学生科技创新项目(2018YA06)。
