摘要
目的研究血府逐瘀汤含药血清对低氧诱导的大鼠肺动脉平滑肌细胞(PASMCs)增殖的影响及其机制。方法低氧诱导大鼠PASMCs增殖,设置正常对照组、低氧模型组、血府逐瘀低剂量组、血府逐瘀高剂量组和NVP-BEZ235组,血府逐瘀低、高剂量组含药血清浓度分别为5%和20%。运用CCK-8法检测不同低氧时间和药物干预对于PASMCs增殖的影响,Western Blot检测细胞p-Akt、p-mTOR和PCNA表达。结果低氧培养36 h时PASMCs OD值最高,且高于常氧培养(P<0.01);与正常对照组比较,低氧模型组OD值明显升高(P<0.01),p-AKT、p-mTOR、PCNA表达增加(P<0.05);与低氧模型组比较,血府逐瘀高剂量组和NVP-BEZ235组OD值不同程度降低(P<0.01),各药物组p-AKT、p-mTOR、PCNA表达减少(P<0.05);与血府逐瘀低剂量组比较,NVP-BEZ235组p-AKT、p-mTOR、PCNA表达减少(P<0.05)。结论血府逐瘀汤含药血清可以抑制低氧诱导的大鼠PASMCs增殖,可能通过阻断PI3K/Akt/mTOR信号通路活化并下调PCNA表达而发挥作用。
Objective To investigate the effect of Xuefu Zhuyu Decoction containing serum on proliferation of rat pulmonary artery smooth muscle cells(PASMCs)induced by hypoxia and its related mechanism.Methods Hypoxia was used to induce PASMCs proliferation.Normal group,hypoxic model group,Xuefu Zhuyu low-dose group,Xuefu Zhuyu high-dose group and NVP-BEZ235 group were set up,with the serum concentrations of Xuefu Zhuyu high-dose group and low-dose group at 5%and 20%,respectively.The effects of different hypoxia time and drug intervention on PASMCs proliferation were determined by CCK-8 assay.The expressions of p-Akt,p-mTOR and PCNA were detected by Western Blot.Results The optical density(OD)of PASMCs was highest after hypoxia culture for 36 h,which was significantly higher than that under normoxia culture(P<0.01).Compared with the normal group,OD and the expressions of p-AKT,p-mTOR and PCNA increased in the hypoxic model group(P<0.01,P<0.05).Compared with the hypoxic model group,OD decreased in the Xuefu Zhuyu high-dose group and the NVP-BEZ235 group(P<0.01),and the expressions of p-AKT,p-mTOR and PCNA decreased in all drug groups(P<0.05).Compared with the Xuefu Zhuyu low-dose group,the expressions of p-AKT,p-mTOR,and PCNA decreased in NVP-BEZ235 group(P<0.05).Conclusion Xuefu Zhuyu Decoction containing serum can inhibit hypoxia-induced proliferation of PASMCs,and the effect is mediated by suppressing the activation of PI3K/Akt/mTOR signaling pathway and down-regulating the expression of PCNA.
作者
李敏静
郭莉
陈晔
严洁萍
董雷
蔡宛如
LI Min-jing;GUO Li;CHEN Ye;YAN Jie-ping;DONG Lei;CAI Wan-ru(Department of Respiratory Medicine,Second Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310005;Department of Pharmacy,Zhejiang Provincial People’s Hospital.Hangzhou 310014)
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2020年第7期836-841,共6页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.81503521)
浙江省自然科学基金资助项目(No.LQ16H310003)。
