摘要
三氯生是一种广谱抗菌剂,广泛应用于日用化学品之中。长期以来,三氯生随生活污水排放至水环境中,对动植物产生毒性作用,进一步通过食物链对人类健康产生威胁。开发高效绿色的三氯生降解方法具有重要的现实意义。微藻作为重要的水环境分解者,参与水体中外源化学物的代谢降解。茉莉酸是一种常见的植物激素,能诱导高等植物对环境胁迫的抗逆性,但其对微藻类低等植物防御体系的影响还未见报道。因此,本文中,笔者考察了不同浓度茉莉酸处理对莱茵衣藻应答三氯生胁迫作用及对三氯生代谢的影响。结果发现,0.05、0.15和0.45μmol/L茉莉酸处理不同程度缓解了三氯生对莱茵衣藻的氧化胁迫,但高浓度茉莉酸(0.45μmol/L)会抑制莱茵衣藻的生长。与三氯生单独处理的莱茵衣藻样本相比,0.15μmol/L茉莉酸处理显著提高了谷胱甘肽S转移酶和多酚氧化酶活力,降低了体内过氧化物酶活力。此外,茉莉酸处理莱茵衣藻体内三氯生积累量显著小于未处理组,而培养液中三氯生残留量无显著差异,说明茉莉酸促进了衣藻对三氯生的代谢降解。进一步通过高分辨质谱鉴定了6种三氯生在莱茵衣藻体内的代谢产物,其中有4种只在茉莉酸处理组中检出,说明在茉莉酸处理下莱茵衣藻代谢三氯生的方式更加多样化,降解效率更高。
Triclosan(TCS)is a broad-spectrum antibacterial agent and widely used in daily necessities and hygiene products.Triclosan residues with the sewage discharged into water environments have toxic effects on animals and plants,as well as potential health risks via the food chain.Thus,the development of effective and eco-friendly degradation method is meaningful.Microalgae,as an important decomposer in water environments can degrade many exogenous chemicals.Jasmonates(JA),a common phytohormone,is reported to induce the resistance to environmental stresses in higher plants.However,the effect of JA on the defense system of lower plants is rarely reported,such as microalgae.Here,we investigated the effect of JA with various concentration on the response to the stress and the degradation of triclosan in Chlamydomonas reinhardtii.The oxidative stress of triclosan was alleviated with the treatments of 0.05,0.15 and 0.45μmol/L of JA exposures,respectively.JA of high concentration inhibited the growth of Chlamydomonas reinhardtii.Compared to the sample with triclosan-treatment alone,the activities of glutathione S-transferase and polyphenol oxidase were enhanced by 0.15μmol/L JA.Peroxidase activity decreased in Chlamydomonas reinhardtii with JA treatment(0.15μmol/L).Additionally,the accumulation of triclosan in algae with JA exposure was significantly lower than that with triclosan treatment alone,indicating that JA prompted the degradation of triclosan in algae.Our result suggests that triclosan degraded under JA exposure with higher efficiency.
作者
张晨怡
毛佳昊
苗馨文
刘汀
熊晓辉
卢一辰
ZHANG Chenyi;MAO Jiahao;MIAO Xinwen;LIU Ting;XIONG Xiaohui;LU Yichen(College of Food Science and Light Industry,Nanjing Tech University,Nanjing 211800,China)
出处
《生物加工过程》
CAS
2020年第4期484-491,共8页
Chinese Journal of Bioprocess Engineering
基金
国家重点研发计划(2018YFC1602803)
国家自然科学基金青年基金(21707070)
江苏省自然科学基金青年基金(BK20170998)。
关键词
三氯生
茉莉酸
莱茵衣藻
解毒
生活污水
triclosan
jasmonic acid
Chlamydomonas reinhardtii
detoxification
sewage
