摘要
目的探讨结直肠癌患者组织中miR-21表达与其临床病理特征的相关性。方法对手术切除的78例结直肠癌患者的组织标本(病灶组)与癌旁组织标本(癌旁组),采用RT-PCR方法检测两组标本中miR-21表达情况,分析其临床病理特征并进行相关性分析。结果病灶组的miR-21相对表达水平显著高于癌旁组(P<0.05)。在病灶组中miR-21相对表达水平与患者年龄、性别、肿瘤部位、病理类型等无相关性(P>0.05),与临床分期、肿瘤直径、浆膜浸润、组织学分化程度等有显著相关性(P<0.05)。logistic分析显示:临床分期、肿瘤直径、浆膜浸润为影响患者miR-21相对表达水平的主要因素(P<0.05)。结论结直肠癌患者组织中miR-21呈现高表达状况,与患者的临床分期、肿瘤直径、浆膜浸润、组织学分化程度等临床病理特征显著相关。
Objective To investigate the relationship between miR-21 expression and clinicopathological features in colorectal cancerpatients.Methods 78 patients with colorectal cancer of tissue specimens(lesion group)and para-cancer tissue samples(para-cancer group)were selected.RT-PCR were used to detect the expression of miR-21 in the 2 groups,and the clinicopathological features of the patients were investigated and given correlation analysis.Results The relative expression of miR-21 in the lesion group was significantly higher than that of the para-cancer group(P<0.05).In the lesion group,the relative expression level of miR-21 was not correlated with the age,gender,tumor location,pathological type,etc.(P>0.05),and was significantly correlated with clinical stage,tumor diameter,serosal invasion,and histological differentiation(P<0.05).Logistic analysis showed that clinical stage,tumor diameter,and serosal infiltration were the main factors affecting the relative expression of miR-21 in patients(P<0.05).Conclusion The expression of miR-21 in tissues of colorectal cancer patients is highly expressed,which is significantly related to the clinical pathological features such as clinical stage,tumor diameter,serosal invasion and histological differentiation.
作者
王哲
马师琦
张建勋
钟丰贺
刘铁权
WANG Zhe;MA Shiqi;ZHANG Jianxun(Panjin Central Hospital,Panjin,124000)
出处
《实用癌症杂志》
2020年第7期1052-1055,共4页
The Practical Journal of Cancer
基金
国家卫生计生委医药卫生科技发展研究中心课题项目(W2015QJ136)。
