摘要
本试验旨在研究胚蛋给养N-乙酰-L-谷氨酸(NAG)对拉萨白鸡种蛋孵化性能以及鸡胚生长、肠道形态的影响。选取17.0胚龄拉萨白鸡活胚蛋240枚,随机分为对照组和NAG给养组,每个组6个重复,每个重复20枚活胚蛋。17.5胚龄时,NAG给养组经羊膜腔无菌注射1.5%NAG营养液,注射剂量为0.1 mL,即NAG补给量为1.5 mg/枚;对照组种蛋不做处理。在19.5胚龄观测鸡胚生长指标、肠道形态,出壳当天统计孵化性能。结果表明:1)孵化后期胚蛋给养NAG对拉萨白鸡鸡胚生长无显著影响(P>0.05)。2)NAG给养组种蛋孵化率显著高于对照组(P<0.05),健雏率2组间无显著差异(P>0.05)。3)与对照组相比,胚蛋给养NAG显著降低了19.5胚龄鸡胚十二指肠火箭形绒毛比例(P<0.05),对空肠和回肠各类型绒毛比例无显著影响(P>0.05)。4)与对照组相比,胚蛋给养NAG显著提高了19.5胚龄鸡胚回肠手指形绒毛高度(P<0.05),对回肠手指形绒毛宽度无显著影响(P>0.05);NAG给养组19.5胚龄鸡胚十二指肠、空肠手指形绒毛高度和宽度较对照组无显著差异(P>0.05)。由此可见,孵化后期(17.5胚龄)经由羊膜腔给养1.5 mg/枚NAG可提高拉萨白鸡种蛋的孵化率,对鸡胚生长未产生显著影响,但可促进鸡胚肠道发育。
The current study was conducted to investigate the effects of in ovo feeding(IOF)of N-acetyl-Lglutamate(NAG)on hatching performance of hatching eggs,growth and intestinal morphology of chicken embryos for Lhasa white chickens.A total of 240 Lhasa white chicken embryo eggs aged 17 d of embryogenesis were randomly allotted to control group and NAG-injection group.Each group contained 6 replicates with 20 embryo eggs each.Embryo eggs in the control group were not injected.Embryo eggs in the NAG-injection group were injected with 0.1 mL of 1.5%NAG nutrient solution at 17.5 d of embryogenesis,that was NAG supplementary amount was 1.5 mg/egg.The growth and intestinal morphology of chicken embryos at 19.5 d of embryogenesis were determined,and the hatching performance was calculated when eggs hatched out.The results showed as follows:1)IOF of NAG did not affect the growth of chicken embryos(P>0.05).2)The hatchability of hatching eggs of NAG-injected group was significantly higher than that of control group(P<0.05),but the rate of healthy chicks had no significant difference between NAG-injected group and control group(P>0.05).3)Compared with the control group,IOF of NAG significantly decreased the proportion of rocket-shaped villi in duodenum of chicken embryos at 19.5 d of embryogenesis(P<0.05),but had no significant effects on the proportions of different types of villus in jejunum and ileum(P>0.05).4)Compared with the control group,IOF of NAG significantly increased the height but not the width of finger-shaped villi in ileum of chicken embryos at 19.5 d of embryogenesis(P<0.05),and had no significant effects on the height and width of finger-shaped villi in duodenum and jejunum(P>0.05).It is concluded that IOF of NAG(1.5 mg/egg)via amniotic injection at the late stage of incubation(17.5 d of embryogenesis)can increase the hatchability of hatching eggs for Lhasa white chickens,and promote the intestinal development without affecting the growth of chicken embryos.
作者
王继光
宋志刚
武书庚
王晶
齐广海
张海军
冯静
马雪英
WANG Jiguang;SONG Zhigang;WU Shugeng;WANG Jing;QI Guanghai;ZHANG Haijun;FENG Jing;MA Xueying(Key Laboratory of Feed Biotechnology of Ministry of Agriculture&Rural Affairs,National Engineering Research Center of Biological Feed,Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China;College of Animal Science and Technology,Shandong Agricultural University,Tai’an 271018,China;Institute of Animal Husbandry and Veterinary,Tibet Academy of Agricultural and Animal Husbandry Science,Lhasa 850000,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2020年第7期3135-3142,共8页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
现代农业产业技术体系建设专项资金(CARS⁃40⁃K12)
北京市自然科学基金(6202029)
国家重点研发计划(2018YFD0500403)。
关键词
胚蛋给养
N-乙酰-L-谷氨酸
孵化性能
胚胎生长
肠道形态
in ovo feeding
N-acetyl-L-glutamate
hatching performance
growth of embryos
intestinal morphology
