摘要
热休克蛋白(heat shock proteins,HSPs)在鱼类的应激与免疫反应中起着重要的作用,HSP90-β是该家族的重要成员。为了探讨澳洲宝石鲈(Scortum barcoo)HSP90-β在免疫应答中的作用,本研究首次克隆得到了澳洲宝石鲈HSP90-β的全长cDNA序列。该序列长2708 bp,其中5’UTR长86 bp,3’UTR长444 bp,ORF长2178 bp,编码725个氨基酸,相对分子质量约为83200,具有5个HSP90家族的特征序列,没有信号肽序列和跨膜结构域,与其他鱼类HSP90-β氨基酸的序列一致性皆超过90%。正常情况下,澳洲宝石鲈HSP90-βmRNA主要在肝脏表达。而感染无乳链球菌(Streptococcus agalactiae)后,其在肝组织中的表达呈先降低后增加的趋势,在脾脏、头肾和脑组织则呈先上升后下降的趋势。这些结果表明澳洲宝石鲈HSP90-β可能参与了无乳链球菌感染引发的免疫反应。
Heat shock proteins(HSPs)play important roles in fish stress and immune response,and HSP90-β is an important member of the HSPs family. In the present study,to investigate the possible role of HSP90-β in the immune response,the full-length cDNA sequence of HSP90-β was first cloned from jade perch(Scortum barcoo). The size of the full-length cDNA of jade perch HSP90-β is 2 708 bp,of which 5’ UTR is 86 bp,3’ UTR is 444 bp,and ORF is 2 178 bp,which encodes 725 amino acids.The molecular weight of jade perch HSP90-β is predicted as 83 200. And this protein owns five characteristic sequences of the HSP90 family. Moreover,it has no signal peptide and transmembrane domain.Phylogenetic analysis showed that the amino acid sequence identity of jade perch HSP90-β is more than90% with those of other fishes. Under normal circumstances,the HSP90-β mRNA of jade perch is mainly expressed in the liver. After infection with Streptococcus agalactiae,the HSP90-β mRNA expression decreased first and then increased in liver. Differently,it increased first and then decreased in spleen,head kidney and brain. This result suggests that the HSP90-β may be involved in the immune response elicited by S. agalactiae infection in jade perch.
作者
高凇泽
孙彩云
吴金英
叶航宇
李文笙
GAO Songze;SUN Caiyun;WU Jinying;YE Hangyu;LI Wensheng(Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals/Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish/School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China)
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第4期7-18,共12页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
现代农业产业技术体系专项资金项目
国家特色鱼淡水鱼产业技术体系(CARS-46)
现代农业人才支撑计划项目(2016-2020)。
