摘要
以带芽的藤茶(Ampelopsis grossedentata)茎段为试验材料,研究藤茶组织培养过程中各个阶段的培养基和激素配比以及炼苗的基质配比。结果表明,藤茶组培苗的快速繁殖体系中,最佳初代培养基为MS+1.5 mg/L 6-BA+0.2 mg/L NAA+2 g/L PVP;初代茎段培养愈伤组织的最佳基质配比为MS+4.0 mg/L 6-BA+2.0 mg/L ZT+1.0 mg/L NAA;藤茶芽的最佳增殖培养基为MS+2.0 mg/L 6-BA+1.0 mg/L NAA+2.0 mg/L ZT;最佳壮苗培养基为MS+1.0 mg/L 6-BA+1.0 mg/L NAA;最佳生根培养基为NAA(0.1 mg/L)+IBA(0.5mg/L),生根率可达到86.7%;炼苗阶段最佳移栽基质为泥炭+珍珠岩(2∶1),并浇以改良霍格兰营养液,炼苗和移栽成活率可达73%。
Stems with buds in Ampelopsis grossedentata as test materials,culture medium and hormone combination in various stages in the process of cultivation of Ampelopsis grossedentata tissue and ratio of matrix in the seedling process were studied. Conclusions are as follows:The optimal primary medium in rapid propagation system of tissue cultured seedling of Ampelopsin Grossedentata was MS+1.5 mg/L 6-BA+0.2 mg/L NAA+2 g/L PVP. The optimal matrix matching for primary stem cultured callus was MS+4.0 mg/L 6-BA+2.0 mg/L ZT+1.0 mg/L NAA. The optimal proliferation medium for Ampelopsin Grossedentata bud was MS+2.0 mg/L 6-BA+1.0 mg/L NAA+2.0 mg/L ZT. The optimal strong seedling medium was MS+1.0 mg/L 6-BA+1.0 mg/L NAA. The optimal rooting medium was NAA(0.1 mg/L)+IBA(0.5 mg/L),with the rooting rate reaching 86.7%. The optimal transplantation matrix at hardening-seedling stage was peat + perlite(2∶1),and the hardening-seedling and transplanting survival rate could reach 73% if it was watered with modified Hoagland nutrient solution.
作者
廖璐婧
江念
孙紫薇
LIAO Lu-jing;JIANG Nian;SUN Zi-wei(Institute of Chinese Medicinal Materials,Hubei Academy of Agricultural Sciences,Enshi 445000,Hubei,China;Shayang Lake Wetland Nature Reserve Management Bureau,Shayang 448200,Hubei,China;Hubei Institute for Nationalities,Enshi 445000,Hubei,China)
出处
《湖北农业科学》
2020年第9期171-176,共6页
Hubei Agricultural Sciences
