摘要
为了解禽源产色葡萄球菌的耐药性,本研究从发病金陵花鸡中分离到1株产色葡萄球菌GZQX2019,并进行细菌分离培养、革兰氏染色镜检、生化试验、16S rDNA序列分析、药敏试验、部分耐药与肠毒素基因检测和动物回归试验。结果显示,分离菌在鲜血琼脂培养基上长出表面凸起、边缘整齐的溶血现象白色菌落;革兰氏染色镜检呈葡萄串状球菌。生化试验结果显示,分离菌蔗糖、精氨酸水解酶、木糖等反应阳性,尿素、木糖醇、麦芽糖等反应阴性。系统进化树显示,分离菌与产色葡萄球菌处于同一分支;药敏试验结果显示,分离菌对红霉素、头孢他啶、多西环素等6种抗菌药耐药,对头孢呋辛、头孢哌酮、新霉素等11种抗菌药敏感。耐药与肠毒素基因检测结果显示,分离菌能检测出热敏感细胞肠毒素基因Alt,耐大环内酯类基因ermB,β-内酰胺类基因VIM、TEM,四环素类基因tetB。动物回归试验结果显示,分离菌对小鼠和雏鸡致病能力不强或者没有致病力。试验成功分离到1株禽源产色葡萄球菌,丰富了禽源产色葡萄球菌的认知。
In order to understand the resistance of avian-origin Staphylococcus chromogenes,a Staphylococcus chromogenes strain named GZQX2019 was isolated from Jinling chicken.The bacteria were isolated and cultured,Gram staining microscopy,biochemical test,16S rDNA sequence analysis,drug sensitivity test,partial resistance and enterotoxin gene detection and animal regression test.The bacteria separation results showed that the white colony with convex surface and neat edge appeared on the blood agar medium,and the Staphylococcus was detected by Gram staining.The results of biochemical tests showed that the reactions of sucrose,arginine hydrolase and xylose were positive,while urea,xylitol and maltose were negative.The phylogenetic tree showed that the isolation was in the same branch with Staphylococcus chromogenes.The drug sensitivity test showed the isolation was resistant to 6 kinds of antibacterial such as erythromycin,ceftazidime and doxycycline,and sensitive to 11 kinds of antibacterial such as cefuroxime,cefoperazone and neomycin.The results of drug resistance and enterotoxin gene detection showed that Alt,ermB,VIM,TEM and tetB could be detected.The results of animal regression tests showed that the isolates were not pathogenic or non-pathogenic to mice and chicks.The experiment successfully isolated one avian-origin Staphylococcus chromogenes,which enriched the cognition of avian-origin Staphylococcus chromogenes.
作者
陈广
温贵兰
张喜懿
田浪
杨佰启
CHEN Guang;WEN Guilan;ZHANG Xiyi;TIAN Lang;YANG Baiqi(College of Animal Science, Guizhou University, Guiyang 550025,China;Guizhou Animal Biological Engineering Technology Research Center,Guiyang 550016,China)
出处
《中国畜牧兽医》
CAS
北大核心
2020年第7期2215-2222,共8页
China Animal Husbandry & Veterinary Medicine
基金
2019年度贵州省农业科技支撑计划项目“贵州地方品种鸡ALV快速检测与中药控制关键技术研究”(黔科合支撑[2019]2286号)
贵州大学博士基金“LSm14A分子在新城疫病毒增殖中的作用”(贵大人基合字(2013)12号)
贵州省高层次创新型人才培养(黔财教[2017]号)
贵州省科技平台及人才团队计划(黔科合平台人才[2018]5253)
贵州省研究生教育创新计划项目(GZZ2017002)。
关键词
金陵花鸡
产色葡萄球菌
耐药基因
毒力基因
Jinling chicken
Staphylococcus chromogenes
drug resistance gene
virulence gene
