摘要
用制备的铜掺杂二氧化硅作为吸附剂,富集雪菊提取液中的木犀草苷,测量富集有目标组分吸附剂的近红外光谱,所得光谱经预处理后,用偏最小二乘法建立木犀草苷的定量校正模型并进行验证.结果表明:当铜掺杂二氧化硅的用量为0.25 g、常温振荡20 min时,对木犀草苷吸附率达89.7%;雪菊提取液中的木犀草苷经吸附剂富集后,无需脱附可直接检测;所得近红外光谱经多元散射校正结合一阶导数预处理后,木犀草苷校正模型的预测质量浓度和参考质量浓度间的相关系数为0.975 0,交叉验证均方根误差为0.959 8 mg/L,木犀草苷在1.5~19.5 mg/L的较低质量浓度范围内,预测集的回收率可达82.6%~111.4%.
The prepared copper-doped silica adsorbent was used to enrich luteoloside in Coreopsis tinctoria,and the adsorbents enriched with luteoloside were determined by near-infrared spectroscopy.After the pretreatment of near-infrared spectra,a quantitative correction model of luteoloside was established by partial least square regression method for determination and validation.The results show that the adsorption efficiency is 89.7%when the mass of copper-doped silica adsorbent is 0.25 g and the adsorption is oscillated at room temperature for 20 min.The adsorbents enriched with luteoloside can be directly detected by near-infrared spectra without desorption.After the NIR spectral data are processed with multiple scattering correction and first derivative,the correlation coefficient between the reference mass concentration and the predicted mass concentration of luteoloside is 0.9750,the root mean square error of cross validation is 0.9598 mg/L,within the lower mass concentration range of 1.5—19.5 mg/L,and the recovery rate of luteoloside in prediction set can achieve 82.6%—111.4%in the calibration model.
作者
楚刚辉
姜盼盼
CHU Ganghui;JIANG Panpan(Xinjiang Laboratory of Native Medicinal and Edible Plant Resources Chemistry,College of Chemistry andEnvironmental Science,Kashgar University,Kashgar 844006,Xinjiang Uygur Autonomous Region,China)
出处
《吉林大学学报（理学版）》
CAS
北大核心
2020年第4期1003-1009,共7页
Journal of Jilin University:Science Edition
基金
国家自然科学基金(批准号:21765012)
新疆维吾尔自治区高校科研计划自然科学重点项目(批准号:XJEDU2020I016)。
关键词
木犀草苷
铜掺杂二氧化硅
雪菊
富集
近红外光谱
luteoloside
copper-doped silica
Coreopsis tinctoria
enrichment
near-infrared spectroscopy
