摘要
目的探索不同浓度乙醇对体外培养的人精子形态、前向运动力和DNA碎片指数的影响。方法采用密度梯度离心法处理精液后将回收的30份精子混悬于不同乙醇浓度的精子培养液中,其中对照组0 mg/dl(A组),实验组(低浓度)20 mg/dl(B组),C组(中浓度)80 mg/dl和D组(高浓度)160 mg/dl。6h后检测各组正常形态精子比例、前向运动精子以及精子DNA碎片指数。结果B组正常形态精子(9.60±2.13)%与A组(10.23±2.48)%相比,差异无统计学意义(P=0.128)。C组(8.03±1.96)%和D组(5.23±1.23)%正常形态精子低于A组,差异有统计学意义(P=0.011,0.000)。前向运动精子比例依次下降[A组(73.03±2.83)%,B组(63.13±4.22)%,C组(53.03±3.05)%,D组(45.07±2.97)%],差异有统计学意义(F=283.454,P=0.000)。D组精子DFI(20.30±5.06)%,高于A组(12.37±3.68)%、B组(12.67±3.78)%和C组(15.70±4.06)%,差异均有统计学意义(P=0.000,0.000,0.001),B组、C组精子DFI高于A组,但差异均无统计学意义(P=0.804,0.339),B组与C组精子DFI比较,差异亦无统计学意义(P=0.478)。结论乙醇可降低体外培养精子的运动力、正常形态精子的比例,升高精子DNA碎片指数。
Objective To explore the effects of different concentration alcohol on motility,morphology and DNA fragment index of spermatozoa cultured in vitro.Methods Thirty spermatozoa samples were prepared by density gradient centrifugation and the recovered spermatozoa was suspended in the culture medium with different concentration of alcohol.The control group with concentration of alcohol 0 mg/dl was named as group A,the experimental groups with concentration of alcohol 20mg/dl,80mg/dl and 160mg/dl were named as group B(low concentration),group C(medium concentration)and group D(high concentration).6 hours later,the ratio of progressive motility,normal morphology and spermatozoa DNA fragmention index(DFI)were detected in each group.Results There was no significant difference(P=0.128)between group B(9.60±2.13)%and A(10.23±2.48)%about the ratio of normal morphology spermatozoa,but the ratio of normal morphology spermatozoa in group C(8.03±1.96)%and group D(5.23±1.23)%were significantly lower than that in group A(P=0.011,0.000).The ratio of progressive motility[group A(73.03±2.83)%,group B(63.13±4.22)%,group C(53.03±3.05)%,group D(45.07±2.970%)]decreased and the difference was statistically significant(F=283.454,P<0.05).Spermatozoa DNA fragmentation index(DFI)of group D(20.30±5.06)%was higher than that of group A(12.37±3.68)%,group B(12.67±3.78)%and group C(15.70±4.06)%,and the difference was statistically significant(P=0.000,0.000,0.001).DFI in group B and group C was higher than that in group A,but the difference was not statistically significant(P=0.804,0.339).Also,there was no significant difference between groups B and group C(P=0.478).Conclusion Alcohol can reduce the ratio of progressive motility and normal morphology sperm and increase sperm DFI.
作者
许鹏宇
李冬秀
郭瑞娟
程立立
XU Peng-yu;LI Dong-xiu;GUO Rui-juan;CHENG Li-li(Reproductive Medicine Center,the Third Hospital of Hebei Medical University,Shijiazhuang 050051,China)
出处
《现代检验医学杂志》
CAS
2020年第3期97-99,102,共4页
Journal of Modern Laboratory Medicine
基金
河北省卫健委项目(No:20190623)。
关键词
乙醇
正常形态精子
前向运动
精子DNA碎片指数
体外培养
alcohol
normal morphology spermatozoa
progressive motility
spermatozoa DNA fragmentation index
in vitro culture
