摘要
目的:基于"温病在下其郁热",探讨大黄在新型冠状病毒肺炎(COVID-19)治疗中的作用及潜在作用机制。方法:使用网络药理学方法,在TCMSP数据库获取大黄的活性成分和作用靶点,并通过Uniprot数据库将获得的靶蛋白名称转换成Gene Symbol;使用Cytoscape3.7.1软件,构建大黄的"化学成分-作用靶点"网络;使用STRING数据库构建作用靶标的蛋白互作网络,并导入Cytoscape中进行网络优化,再通过"MCODE"插件进行聚类分析;使用"ClueGo"插件对大黄的核心靶标进行GO功能分析和KEGG通路富集分析,结果使用R软件进行可视化。结果:共筛选得到β-谷甾醇、芦荟大黄素等7个有效化合物,对应靶标49个;靶标PPI网络聚类分析得到2个子网络,涉及JUN、CASP3、IL-1β、CASP8、CASP9等18个靶标;GO分析得到G蛋白偶联胺受体活性、核受体的活动、配体激活的转录因子活性、组蛋白磷酸化和类固醇激素受体活性等296个生物学功能(P<0.01);KEGG富集得到p53信号通路、IL-17信号通路、VEGF信号通路、c型凝集素受体信号通路和TNF通路等31条通路(P<0.01)。结论:大黄可以通过多成分-靶点-通路的途径,发挥抗病毒、抗炎、抗氧化应激、抗细胞凋亡和调节机体免疫等功能,从而起到治疗COVID-19的作用。
Objective:To explore the effect and potential mechanism of rhubarb on COVID-19 based on the theory of"Wenbingzaixiayiyure".Methods:Based on network pharmacology,the chemical components and targets of rhubarb were obtained in the TCMSP database,and the name of the target protein was converted into Gene Symbol through the Uniprot database.The"chemical composition-target"network of rhubarb was constructed by Cytoscape3.7.1 software.The target protein interaction network was built by using STRING database,and was imported into Cytoscape for network optimization,then"MCODE"plug-in was used to cluster analyze.GO function analysis and KEGG pathway enrichment analysis were performed by using the"ClueGo"plug-in,the results were visualized by R software.Results:7 chemical components including beta-sitosterol and aloe-emodin were screened,as well as 49 corresponding targets.2 subnetworks involving 18 targets,such as JUN,CASP3,IL1 B,CASP8 and CASP9,were revealed by cluster analysis of PPI network of target.296 biological functions including G protein-coupled amine receptor activity,nuclear receptor activity,ligand-activated transcription factor activity,histone phosphorylation and steroid hormone receptor activity were revealed by GO function analysis(P<0.01).31 pathways including p53 signaling pathway,IL-17 signaling pathway,VEGF signaling pathway,C-type lectin receptor signaling pathway and TNF signaling pathway were revealed by KEGG pathway enrichment analysis(P<0.01).Conclusion:Rhubarb can be used to treat COVID-19 for its effects of anti-virus,anti-inflammation,anti-oxidative stress,anti-apoptosis and regulating the body immunity,through a multi-component-target-pathway way.
作者
许禄华
黄小靖
李彦荣
宋银枝
曾志聪
刘卓超
周旭
林丰夏
Xu Luhua;Huang Xiaojing;Li Yanrong;Song Yinzhi;Zeng Zhicong;Liu Zhuochao;Zhou Xu;Lin Fengxia(Shenzhen Bao'an Traditional Chinese Medicine Hospital Group,the Affiliated Hospital of Guangzhou University of Chinese Medicine,Shenzhen 518133;Graduate school,Guangzhou University of Chinese Medicine,Guangzhou 510405)
出处
《中药药理与临床》
CAS
CSCD
北大核心
2020年第2期85-90,共6页
Pharmacology and Clinics of Chinese Materia Medica
基金
2019年国家中医药管理局(编号:2019 GZY-FJS-2019-004)
深圳市科技创新计划项目(JCYJ20170306152620264)。
关键词
大黄
新型冠状病毒肺炎
温病
下法
网络药理学
机制
Rhubarb
COVID-19
"Wen"diseases
"Xia"therapy
Network pharmacology
Mechanism
