摘要
为研究不同培养条件对紫色秃马勃(Calvatia lilacina)菌丝生长的影响,优选出最适合其菌丝生长的条件。通过单因素试验,以菌丝的生长状态和生长速率为指标,筛选出最佳碳源、氮源、微量元素、培养温度和初始pH;再通过正交试验L9(3^4),进一步确定最优的培养基配比组成。结果表明:最佳碳源为葡萄糖、最佳氮源为酵母粉、最佳微量元素为MgSO4,最适合紫色秃马勃菌丝生长的培养基配方为葡萄糖浓度2%、酵母粉浓度1%、MgSO4浓度0.1%,最适初始pH值6.5,最适培养温度30℃。通过对紫色秃马勃生物学特性的研究,得到了最适合其菌丝生长的培养条件,为紫色秃马勃的进一步研究和开发利用提供依据。
To study the effects of culture conditions on the mycelial growth of Calvatia lilacina and the conditions for the mycelial growth of Calvatia lilacina.Through single factor experiment,the optimum carbon source/nitrogen source/trace element,incubation temperature and initial pH value were selected according to the growth status and growth speed of mycelia.Then,the optimum medium composition was further determined by orthogonal experiment L9(3^4).The results showed that the selected carbon source/nitrogen source/trace elements are glucose,yeast powder and MgSO4,respectively.The optimized medium for mycelial growth of Calvatia lilacina was 2%glucose concentration,1%yeast powder concentration,0.1%MgSO4 concentration.The optimum initial pH value was 6.5,and the optimum culture temperature was 30℃.Through studying the biological characteristics of Calvatia lilacina,the most suitable culture conditions for its mycelium growth were obtained.The study provided the basis for further research and development of Calvatia lilacina.
作者
穆双双
步洪石
胡德
李小欢
李正龙
王欢
王淑敏
MU Shuangshuang;BU Hongshi;HU De;LI Xiaohuan;LI Zhenglong;WANG Huan;WANG Shumin(College of Pharmacy,Changchun Unirersiry of Traditinal Chinese Medicine,Changchun 130117,China)
出处
《菌物研究》
CAS
2020年第1期31-36,42,共7页
Journal of Fungal Research
基金
国家自然科学基金项目(81274037)。
关键词
药用真菌
生长速率
菌丝形态
碳源
氮源
微量元素
medicinal fungi
growth rate
mycelial morphology
carbon source
nitrogen source
trace element
