摘要
目的:探讨盐酸布比卡因对大鼠L5脊神经结扎术(SNL)引起的神经病理性疼痛的早期阻滞作用。方法:雄性SD大鼠随机分为3组:L5SNL组:包括L5SNL手术组和L5SNL假手术组;L5脊神经背根切断术(DR)组:包括L5DR联合L5SNL组、L5DR假手术联合L5SNL组、L5DR联合L5SNL假手术组;L5背根节(DRG)节外注射盐酸布比卡因组:包括L5SNL术后L5DRG节外盐酸布比卡因注射组或HEPES缓冲液注射组。采用von Frey丝测试各组大鼠术侧及对侧后肢足底L4脊神经支配皮肤区域特异性的疼痛反应;用免疫荧光组织化学染色技术检测大鼠L5脊髓节段Iba-1的表达。结果:L5SNL术后大鼠术侧50%爪缩阈值(PWT)降低(P<0.05),并维持至第14 d;成功建立L5SNL疼痛模型。L5DR与L5SNL联合作用下,术侧50%PWT降低(P<0.05),其疼痛阈值水平及时程变化与仅进行L5SNL手术无差异(P>0.05)。L5SNL术后L5DRG节外盐酸布比卡因注射组和HEPES缓冲液注射组的大鼠术侧50%PWT均降低(P<0.05),但盐酸布比卡因注射组降低程度明显小于HEPES缓冲液注射组(P<0.05),且术侧脊髓第5腰椎节段内小胶质细胞基本无活化,与正常对照大鼠相比,无统计学差异(P>0.05),但与HEPES缓冲液注射组有明显差异(P<0.05)。结论:L5DR并不能阻断或减轻大鼠脊神经损伤引起的神经病理性疼痛,而L5DRG节外注射盐酸布比卡因可有效阻滞大鼠脊神经损伤引起的神经病理性疼痛行为反应和相应脊髓节段小胶质细胞的活化。
Objective:To investigate the early blocking effects of bupivacaine hydrochloride on neuropathic pain induced by L5 spinal nerve ligation(SNL)in rat.Methods:Male SD rats were randomly divided into 3 groups:L5SNL group:including L5SNL and L5SNL sham;L5DR group:including L5 dorsal rhizotomy(DR)combined with L5SNL,L5DR sham combined with L5SNL and L5DR combined with L5SNL sham;L5 dorsal root ganglia(DRG)injection of bupivacaine hydrochloride group:including injection of bupivacaine hydrochloride and injection of HEPES buffer to L5DRG after L5SNL.For ipsilateral and contralateral rats hindpaws of each group,the von Frey hair behavior test method was used to detect the specific pain response of the skin area innervated by the L4 spinal nerve.The expression of Iba-1 in L5 spinal cord segment in rat was detected by immunofluorescence histochemincal technique.Results:After L5SNL,the ipsilateral 50%paw withdrawal threshold(PW T)of rats decreased(P<0.05)and was maintained until the 14 th day.The model,L5SNL,was successfully established.After the combined action of L5DR anti L5SNL,the ipsilateral 50%PWT of rats decreased(P<0.05),and its pain threshold level and time course were no difference with that of rats with L5SNL surgery alone.After L5SNL,the ipsilateral 50%PWT decreased(P<0.05)both in rats injected with bupivacaine hydrochloride or HEPES buffer to the L5DRG,but the reduction degree of bupivacaine hydrochloride injection group was lower than the HEPES buffer injection group(P<0.05).And the microglia were not activated in the 5th lumbar segment of the ipsilateral spinal cord in the bupivacaine hydrochloride injection group,showing no difference with normal animals as control(P>0.05)but difference with HEPES buffer injection group(P<0.05).Conclusion:L5DR cannot block or alleviate neuropathic pain caused by L5 spinal nerve injury in rats,while L5DRG injection of bupivacaine hydrochloride can effectively block the neuropathic pain behavioral response and the activation of microglia in corresponding spinal cord segment cau
作者
唐昕桐
卫宇菲
王蕾
Tang Xintong;Wei Yufei;Wang Lei(Department of Histology,Embryology and Neurobiology,West China School of Preclinical and Forensic Medicine,Chengdu 610041,China)
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2020年第3期247-252,共6页
Chinese Journal of Neuroanatomy
关键词
背根切断
脊神经结扎术
背根节
盐酸布比卡因
神经病理性疼痛
大鼠
dorsal rhizotomy
spinal nerve ligation
dorsal root ganglion
bupivacaine hydrochloride
neuropathic pain
rat
