摘要
目的通过对比分析采用多水囊叠加加压法制备腹腔高压的"定容"动物模型与气体灌注法制备的"定压"动物模型,筛选更符合临床腹腔间隔室综合征(ACS)病变规律的动物模型制备方法。方法选择10只健康家猪,按随机数字表法分为两组,每组5只。分别采用水囊叠加加压法制备腹腔高压"定容模型"(定容模型组)和采用人工气腹法制备腹腔高压"定压模型"(定压模型组),两组腹腔压力均为25 mmHg(1 mmHg=0.133 kPa),制模后观察4 h,每小时测压1次,测压结果取均值,绘制腹腔压力-时间变化曲线。制模后4 h处死动物取心脏和肺脏,用苏木素-伊红(HE)染色,镜下观察组织病理学改变。结果两组实验猪均成功制模。定容模型组术后0、1、2、3、4 h腹腔压力值逐渐升高(mmHg:分别为25.0±0、27.1±0.2、29.4±0.1、30.9±0.2、33.1±0.1),腹腔压力与时间呈正相关关系(函数方程:Y1=25.1020+1.9960X1,R2=0.9962,P=0.0001)。定压模型组术后所有时间点腹腔压力值均维持在25 mmHg,腹腔压力与时间无线性相关关系(函数方程:Y2=25)。HE染色显示,定容模型组心肌纤维伴玻璃样变性,横纹明显减少,部分心肌纤维萎缩,可见细胞核聚集;肺组织可见出血,有慢性炎性细胞浸润及炎性渗出。定压模型组心肌纤维部分萎缩,部分肥大,局灶可见玻璃样变性,局灶横纹消失,心肌间动脉扩张充血;肺泡上皮部分区域轻度增生,可见心衰细胞,支气管动脉可见扩张充血,管腔内见大量红细胞及均匀一致的淡染物质。结论多水囊叠加加压法制备腹腔高压"定容"动物模型,在制模后腹腔压力随疾病的发展持续动态升高,符合临床ACS病变规律,比"定压"动物模型更适合科研时制备腹腔高压动物模型。
Objective To select the animal model more consistent with the pathophysiology of abdominal compartment syndrome(ACS)through the comparative study of the methods of multiple water sacs superimposed compression and gas perfusion.Methods Ten experimental pigs were randomly divided into two groups(n=5):the"constant volume model"(constant volume model group)and the"constant pressure model"(constant pressure model group)of intra-abdominal hypertension.The models were prepared by the method of water sac superposition and pressurization,and artificial pneumoperitoneum respectively.The abdominal pressures of both groups were 25 mmHg(1 mmHg=0.133 kPa)and observed for 4 hours.The pressure was measured once an hour for 4 hours and the pressure-time curves of the two groups were drawn respectively.The experimental animals were sacrificed 4 hours after modeling.The heart and lung were harvested,and the histopathological changes were observed by hematoxylin-eosin(HE)staining.Results Two groups of experimental pigs were successfully modeled.The abdominal pressure gradually increased at 0,1,2,3,4 hours after operation in the constant volume model group(mmHg:25.0±0,27.1±0.2,29.4±0.1,30.9±0.2,33.1±0.1),and there was a positive correlation between the abdominal pressure and time(functional equation:Y1=25.1020+1.9960X1;R2=0.9962,P=0.0001).The abdominal pressure value in the constant pressure model group at 0,1,2,3,4 hours were maintained 25 mmHg,and there was no linear correlation between the abdominal pressure and time(functional equation:Y2=25).HE staining showed that in the constant volume model group,the myocardial fibers were accompanied with hyaline degeneration,significantly reduced transverse lines,part of myocardial fiber atrophy,and visible nuclear aggregation;hemorrhage,chronic inflammatory cell infiltration and inflammatory exudation were found in the lung tissues.In the constant pressure model group,partial atrophy of myocardial fiber,partial hypertrophy,focal hyaline degeneration,disappearance of local striae,hyali
作者
陈勇
王宏业
杨秀峰
齐凤龙
乔进余
张盼盼
Chen Yong;Wang Hongye;Yang Xiufeng;Qi Fenglong;Qiao Jinyu;Zhang Panpan(Department of Infection,the Affiliated Hospital of Shanxi Datong University,Datong 037005,Shanxi,China;Department of Tumor Surgery,the Affiliated Hospital of Shanxi Datong University,Datong 037005,Shanxi,China)
出处
《中华危重病急救医学》
CAS
CSCD
北大核心
2020年第4期498-501,共4页
Chinese Critical Care Medicine
基金
山西省重点研发计划(指南)项目(201603D321054)。
关键词
腹腔高压
动物模型
水囊叠加加压法定容模型
人工气腹法定压模型
容积
压力-时间曲线
Intra-abdominal hypertension
Animal model
The model of water sac superposition and pressurization
The model of artificial pneumoperitoneum
Volume
Pressure-time curve
