摘要
为筛选结直肠癌MTX耐药的潜在靶点,运用生物信息学手段从GEO(Gene expression omnibus)收集并整理有关结直肠癌甲氨蝶呤(MTX)耐药相关数据,利用GEO2R筛选对照细胞和耐药细胞表达差异的基因,并用细胞生物学手段验证该基因是否参与MTX耐药.结果显示15个基因在MTX耐药结直肠癌细胞中差异表达,其中13个上调,2个下调;其中表达差异最显著的是SPINK1.通过细胞增殖实验、细胞毒性实验证实敲低SPINK1抑制细胞增殖,解除结直肠癌甲氨蝶呤耐药性.该研究表明SPINK1可能是结直肠癌耐药潜在的治疗靶点.利用细胞生物学方法进一步确认其功能,可为进一步实验验证提供依据.
To screen potential targets of colorectal cancer and explore the pathogenesis of lung adenocarcinoma by bioinformatics,gene Expression Omnibus(GEO)was used to collect and collate data on genes in colorectal cancer.the differentially expressed genes was screened byGEO2R,and their functions were detected by cell biology methods.15 genes were differentially expressed in CRC,of which 13 were up-regulated and 2 were down-regulated.The most significant difference was SPINK1.SPINK1 may be a potential therapeutic target for MTX-resistant colorectal cancer.Bioinformatics can effectively screen target genes and provide basis for further experimental verification.
作者
李鹏
杨景文
孙轶
孟令祥
秦海
张锡鹏
Li Peng;Yang Jingwen;Sun Yi;Meng Lingxiang;Qin Hai;Zhang Xipeng(Department of Anorectal,Tianjin People's Hospital,Tianjin 300121,China)
出处
《南开大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第3期58-61,共4页
Acta Scientiarum Naturalium Universitatis Nankaiensis
