摘要
目的探讨四溴双酚A(tetrabromobisphenol A,TBBPA)对大鼠肝脏的损伤效应及其作用机制,以期为TBBPA生态健康风险评价提供理论依据。方法选用雄性SD大鼠,设置0、10、100、1 000 mg/kg TBBPA 4个处理组和0(R) mg/kg、1 000(R) mg/kg TBBPA 2个恢复组。对6组大鼠连续灌胃35 d,2个恢复组停止灌胃后正常进食,自然恢复1周。结果连续灌胃35 d后,1 000 mg/kg处理组大鼠的平均体增重[(142. 7±24. 8) g]比0 mg/kg组[(218. 5±33. 3) g]减少了34. 7%,差异有统计学意义(P<0. 05);1 000 mg/kg组大鼠肝脏细胞边界模糊,空泡化程度加剧;与0 mg/kg组比较,1 000 mg/kg组大鼠肝脏中活性氧(ROS)含量下降45. 0%,还原型谷胱甘肽(GSH)含量上升27. 3%,谷胱甘肽过氧化物酶(GSH-PX)活性上升44. 5%,差异均有统计学意义(P<0. 05)。结论 TBBPA通过对大鼠肝脏中总超氧化物歧化酶(T-SOD)、ROS、GSH和GSH-Px活性的影响,破环大鼠肝脏中氧化还原系统的平衡,造成肝脏的氧化损伤效应,进而抑制其生长发育。
Objective To explore the damage effect of tetrabromobisphenol A(TBBPA)on rat liver and its mechanism,in order to provide theoretical basis for TBBPA ecological health risk assessment.Methods Male SD rats were selected and four treatment groups of 0,10,100 and 1000 mg/kg TBBPA and two recovery groups of 0(R),1000(R)mg/kg TBBPA were set up.The rats in six groups were given gavage continuously for 35 days,and the rats in two recovery groups were fed normally after stopping gavage and recovered naturally for one week.Results After continuous gavage for 35 days,the average body weight gain of rats in the 1000 mg/kg group((142.7±24.8)g)was 34.7%lower than that in the 0 mg/kg group((218.5±33.3)g).The difference was statistically significant(P<0.05).In the 1000 mg/kg group,the boundary of liver cells was blurred and the degree of vacuolation was increased.Compared with the 0 mg/kg group,ROS decreased by 45.0%,GSH and GSH-Px increased by 27.3%and 44.5%in the 1000 mg/kg group.The differences were statistically significant(P<0.05).Conclusions TBBPA could break the balance of redox system in rat liver by affecting the activities of T-SOD,ROS,GSH and GSH Px,and then cause the oxidative damage effect of liver,and then inhibit its growth and development.
作者
何建波
张杭君
HE Jianbo;ZHANG Hangjun(School of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 311121,China)
出处
《健康研究》
CAS
2020年第3期315-320,F0003,共7页
Health Research
基金
浙江省新苗人才计划(2018R413076)。
