摘要
目的:探讨慢性HBV感染(CHI)不同临床阶段患者外周血T细胞状态,为CHI临床分期和防治提供科学依据。方法:本研究收录CHI患者,按照患者临床症状将其划分为免疫耐受(IT)、免疫活跃(IA)、免疫控制(IC)、再活化组(RA),健康志愿者(HD)为对照组。分离外周血单个核细胞(PBMC),使用多色流式细胞术分析患者外周血CD4^+T细胞、CD8^+T细胞亚群频率和CD8^+T细胞表面PD-1的表达量。结果:与HD相比,CHI组T细胞及亚群变化无显著差异;RA组CD3^+T细胞百分比显著下降。对于CD8^+T细胞亚型,IC和RA组初始CD8^+T细胞(Na ve T cell,Tn)频率下降;IT组效应记忆CD8^+T细胞(Tem)频率降低;而IC组效应CD8^+T细胞(Te)频率显著增高(P<0.05)。相较于高HBV DNA载量组(IT),低HBV DNA载量组(IC和RA)Tn频率降低,Te频率增高(P<0.05);IA组CD8^+T细胞PD-1表达量最高,其次是IC组(P<0.05)。对于CD4^+T细胞亚型,与HD相比,CHI中IT和RA组中枢记忆CD4^+T细胞(Tcm)频率明显升高;各组Treg频率均上升,IA组Treg频率升高较明显(P<0.05),Treg频率与ALT呈正相关(r=0.7436,P=0.0036)。结论:临床诊断指标联合CHI患者T细胞亚型频率变化及免疫抑制功能检测,对慢性HBV感染疾病的分期诊断和治疗以及预测预后转归具有重要参考意义。
Objective:To explore status of peripheral blood T cells in patients with chronic HBV infection(CHI)at different clinical stages,and provide scientific basis for diagnosis of the clinical staging and prevention of CHI.Methods:In the study,patients with chronic HBV infection were enrolled according to the clinical symptoms,these patients were divided into immune tolerant(IT),immune active(IA),immune control(IC),reactivation(RA)group and healthy donor(HD)was the control group.Peripheral blood mononuclear cells(PBMCs)were isolated,and the frequencies of CD4^+T cell subsets,CD8^+T cell subsets,and expression of PD-1 in CD8^+T cell were analyzed using multicolor flow cytometry.Results:Compared with HD,there was no significant difference in T cell subpopulation in the CHI group,the percentage of CD3^+T cell decreased significantly in RA group.For CD8^+T cell subsets,the frequency of naive CD8^+T cells(Tn)decreased significantly in IC and RA group,the frequency of effector memory CD8^+T cells(Tem)decreased significantly in the IT group,while the frequency of effector CD8^+T cells(Te)increased significantly in the IC group(P<0.05).Compared with patients in IT group with a high HBV DNA load,the frequency of Te was significantly increased,while the frequency of Tn was significantly decreased in IC and RA group with a low HBV DNA load(P<0.05).The expression of PD-1 in CD8^+T cell was highest in IA group,followed by IC group(P<0.05).For CD4^+T cell subsets,compared with HD group,the frequency of central memory CD4^+T cell(Tcm)increased significantly in IT and RA group,the frequency of Treg increased in CHI group,the frequency of Treg increased significantly in IA group(P<0.05),and was positively correlated with ALT(r=0.7436,P=0.0036).Conclusion:Clinical diagnostic indicators combined with the frequency of T cell subsets and immunosuppressive function detection in patients with CHI might be considered in diagnosis of the clinical staging and the prediction of prognosis,and for treatment of chronic HBV infection.
作者
罗雅煊
李婷婷
王嘉悦
秦波
金艾顺
LUO Ya-Xuan;LI Ting-Ting;WANG Jia-Yue;QIN Bo;JIN Ai-Shun(Department of Immunology,College of Basic Medical Sciences,Chongqing Medical University,Chongqing 400016,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2020年第12期1494-1498,共5页
Chinese Journal of Immunology
