摘要
目的探讨连接斑珠蛋白(JUP)体外对放疗抵抗子宫颈癌细胞的影响。方法对子宫颈癌细胞株SiHa、HeLa、Me-180采用单次剂量1 Gy、每周3次的60Co放射线照射,诱导细胞耐放疗性,得到获得性放疗抵抗细胞株SiHaIR、HeLaIR、Me-180IR,以相应野生型细胞株作为对照组。分别采用实时荧光定量聚合酶链反应(RT-qPCR)、蛋白质印迹法检测6组细胞中JUP mRNA及蛋白的表达情况,采用细胞划痕实验检测细胞迁移能力。结果SiHa组和SiHaIR组、HeLa组和HeLaIR组、Me-180组和Me-180IR组JUP mRNA相对表达量分别为1.74±0.06和0.48±0.02(t=12.327,P<0.01)、1.77±0.06和0.28±0.03(t=14.698,P<0.01)、2.28±0.06和0.78±0.01(t=7.367,P<0.01),JUP蛋白的相对表达量分别为2.36±0.03和0.55±0.02(t=9.245,P<0.01)、2.13±0.02和0.23±0.01(t=15.643,P<0.01)、1.96±0.05和0.73±0.02(t=5.826,P<0.01)。划痕后培养12、24、48 h时,3组放疗抵抗细胞迁移率均较相应野生型细胞增加,差异均有统计学意义(均P<0.05)。结论JUP在放疗抵抗子宫颈癌细胞株的表达量低于野生型细胞株,且放疗抵抗细胞的迁移能力增强。
Objective To explore the effect of junctional plakoglobin(JUP)on the radiotherapy-resistant cervical cancer cells in vitro.Methods Cervical cancer cell lines SiHa,HeLa and Me-180 were irradiated with 1 Gy of 60Co radioactive rays for 3 times every week to induce the radiotherapy resistance of cells which obtained the radiotherapy-resistant cell lines SiHaIR,HeLaIR and Me-180IR.The corresponding wild-type cell lines were served as control groups.Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)and Western blot were used to detect the expressions of JUP mRNA and protein in the 6 groups of cells.The cell scratch test was used to detect the cell migration ability.Results The relative expressions of JUP mRNA in SiHa and SiHaIR groups,HeLa and HeLaIR groups and Me-180 and Me-180IR groups were the relative expressions of JUP protein were 1.74±0.06 and 0.48±0.02(t=12.327,P<0.01),1.77±0.06 and 0.28±0.03(t=14.698,P<0.01),2.276±0.061 and 0.780±0.011(t=7.367,P<0.01);2.36±0.03 and 0.55±0.02(t=9.245,P<0.01),2.13±0.02 and 0.23±0.01(t=15.643,P<0.01),1.96±0.05 and 0.73±0.02(t=5.826,P<0.01).When culturing the cells for 12,24,and 48 h after scratching,the migration rates in 3 groups of radiotherapy-resistant cell lines were increased compared with the corresponding wild-type cell lines,and the differences were statistically significant(all P<0.05).Conclusion The expressions of JUP in radiotherapy-resistant cervical cancer cell lines are lower than those in wild-type cell lines,and the migration ability of radiotherapy-resistant cells is enhanced.
作者
陈笑
黄巧丽
夏念
陈亦乐
Chen Xiao;Huang Qiaoli;Xia Nian;Chen Yile(Department of Gynecologic Oncology,Hunan Cancer Hospital,Changsha 410013,China)
出处
《肿瘤研究与临床》
CAS
2020年第5期309-312,共4页
Cancer Research and Clinic
关键词
宫颈肿瘤
连接斑珠蛋白
辐射耐受性
Uterine cervical neoplasms
Junction plakoglobin
Radiation tolerance
