摘要
目的探讨白假丝酵母CDR1/CDR2和MDR1基因表达对耐药性及氧化应激的影响。方法收集2016年4月-2017年9月宁波市妇女儿童医院念珠菌性阴道炎患者阴道分泌物中分离获得的白假丝酵母109株,采用微量稀释法测定109株白假丝酵母对氟康唑耐药的最小抑菌浓度(MIC)值,采用PCR法检测白假丝酵母耐药基因CDR1、CDR2、MDR1表达,氧化应激相关基因CAP1、GRP2以及ROS清除相关基因SOD2、SOD5表达情况。结果109株白假丝酵母中65株对氟康唑敏感,44株对氟康唑耐药。耐药株组耐药基因CDR1、CDR2以及MDR1相对表达量均高于敏感组菌株(P<0.05)。耐药株组氧化应激基因CAP1、GRP2相对表达量明显低于敏感组菌株(P<0.05),耐药株组ROS清除相关基因SOD2、SOD5相对表达量明显高于敏感组菌株(P<0.05)。CDR1、CDR2以及MDR1与CAP1、GRP2均呈显著负相关关系(P<0.05),CDR1、CDR2以及MDR1与SOD2、SOD5均呈显著正相关关系(P<0.05)。结论白假丝酵母耐药菌株出现外排泵基因CDR1/CDR2以及MDR1高表达,这可能是白假丝酵母耐药机制之一,此外,CDR1/CDR2以及MDR1基因的高表达可降低白假丝酵母氧化应激反应,增强白假丝酵母对氧化应激的适应性。
OBJECTIVE To explore the impact of expression of CDR1/CDR2 and MDR1 genes in Candida albicans on drug resistance and oxidative stress.METHODS Totally 109 strains of C.albicans were isolated from vaginal secretions of the patients with Candida vaginitis who were treated in Ningbo Women′s and Children′s Hospital from Apr 2016 to Sep 2017,the minimal inhibitory concentration(MIC)of fluconazole resistance against the 109 strains of C.albicans was determined by using microdilution method,the expression of the drug resistance genes CDR1,CDR2 and MDR1 and the oxidative stress-related genes CAP1,CRP2 as well as ROS scavenging genes SOD2 and SOD5 in the C.albicans strains was detected with the use of PCR.RESULTS Among the 109 strains of C.albicans,65 were susceptible to fluconazole,and 44 were resistant to fluconazole.The relative expression levels of the drug resistance genes CDR1,CDR2 and MDR1 were significantly higher in the drug-resistant strains than in the drug-susceptible strains(P<0.05).The relative expression levels of oxidative stress genes CAP1 and GRP2 were significantly lower in the drug-resistant strains than in the drug-susceptible strains(P<0.05).The relative expression levels of ROS scavenging genes SOD2 and SOD5 were significantly higher in the drug-resistant strains than in the drug-susceptible strains(P<0.05).CDR1,CDR2 and MDR1 showed significantly positive correlation with SOD2 and SOD5(P<0.05).CONCLUSION The high expression of efflux pump genes CDR1/CDR2 and MDR1 in the C.albicans strains may be one of the drug resistance mechanisms of the strains,furthermore,the high expression of CDR1/CDR2 and MDR1 genes may reduce the oxidative stress response and boost the strains′adaptability to the oxidative stress.
作者
王雪青
胡敏
孙宛
朱闽波
WANG Xue-qing;HU Min;SUN Wan;ZHU Min-bo(Ningbo Sixth Hospital,Ningbo,Zhejiang 315040,China;不详)
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2020年第9期1297-1301,共5页
Chinese Journal of Nosocomiology
基金
浙江省科研基金资助项目(20183282)。
