摘要
植物多糖是由单糖分子以糖苷键连接聚合而成的复杂长链大分子,具有降血压、降血脂、抗菌、抗氧化、抗肿瘤和免疫调节等生理功能。以冬瓜为原料,采用水提醇沉法提取冬瓜多糖,研究提取温度、提取时间、料液比对冬瓜多糖提取率的影响,通过单因素试验和响应面分析法(RSM)对提取工艺进行优化;研究冬瓜多糖的抗氧化性,利用光电比浊法和滤纸片扩散法测定抑菌效果。研究结果表明,冬瓜多糖的最佳提取工艺参数为:提取温度86.32℃、提取时间2.23 h、提取料液比2.05∶1,在该条件下冬瓜多糖的提取率为0.96%。冬瓜多糖对羟基自由基和1,1-二苯基-2-三硝基苯肼(DPPH)自由基均显示出较强清除能力,冬瓜多糖对大肠杆菌、金黄色葡萄球菌及枯草芽孢杆菌具有一定的抑制作用,以金黄色葡萄球菌最为显著,其最低抑菌浓度(MIC)为5 mg/mL。此研究成果可为冬瓜多糖在医药、食品等行业的潜在应用提供理论基础。
Plant polysaccharides are complex long-chain molecules making up of simple sugar molecules connected by glycosidic linkages,which has such physiological functions as reducing blood pressure and blood lipids,antibacterial activity,anti-oxidation,anti-tumor,immunomodulatory and so on.In this research,polysaccharides from wax gourd was extracted by water extraction and ethanol precipitation and the effects of extraction time,extraction temperature and solid-liquid ratio on the extraction yield of polysaccharides were studied.The extraction process was optimized by single factor test and response surface methodology(RSM).The anti-oxidative activity of wax gourd polysaccharides was studied,and the antibacterial activity was determined by photoelectric turbidimetry and filter paper diffusion method.These results of the optimal extraction conditions were as follow:extraction temperature 86.32℃,extraction time 2.23 h,and solid-liquid ratio 2.05∶1.Under these conditions,the extraction rate of polysaccharides from wax gourd was 0.96%,wax gourd polysaccharides showed strong scavenging ability to hydroxyl radicals and 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH).Wax gourd polysaccharides exhibited resistance to Escherichia coli,Staphylococcus aureus and Bacillus subtilis,especially to Staphylococcus aureus.Their minimal inhibition concentration(MIC)against Staphylococcus aureus was 5 mg/mL.The research results can provide a theoretical basis for the potential application of polysaccharides in medicinal and food industries.
作者
詹亦贝
周楷旋
汪铭轩
胡菲
ZHAN Yibei;ZHOU Kaixuan;WANG Mingxuan;HU Fei(School of Chemistry and Chemical Engineering,Hubei Polytechnic University,Huangshi Hubei 435003)
出处
《湖北理工学院学报》
2020年第3期32-38,共7页
Journal of Hubei Polytechnic University
基金
国家自然科学基金青年项目(项目编号:81600459)
湖北省自然科学基金青年项目(项目编号:2016CFB312)
湖北理工学院引进人才项目(项目编号:15xjz03R)
中央引导地方科技发展专项(项目编号:2019ZYYD046)。
关键词
多糖
提取
响应面分析
抗氧化性
抑菌性
polysaccharides
extraction
response surface methodology
anti-oxidation
antibacterial activity
