摘要
建立芒果(Mangifera indica L.)果肉中总黄酮及芒果苷含量的测定方法,采用紫外-分光光度计法测定总黄酮;采用高效液相色谱法测定芒果苷。结果表明,总黄酮在17~520μg/mL浓度范围内呈现良好线性关系(R^2=0.999 9),平均回收率为99.03%,RSD为1.42%(n=6);芒果苷在4.0~80.0μg/mL浓度范围内呈现良好线性关系(R=0.999 9),平均回收率为101.59%,RSD为1.87%(n=6)。该方法简便、准确度高、重复性良好,可用于芒果果肉中总黄酮及芒果苷的测定。
A method for the determination of total flavonoids and mangiferin in mango flesh was established.The total flavonoids in mango flesh were determined by uv-spectrophotometer.Mangiferin was determined by high performance liquid chromatography.The results showed that the total flavonoids had a good linear relationship in the concentration range of 17~520μg/mL(r=0.9999),the average recovery was 99.03%,and the RSD was 1.42%(n=6).Mangiferin showed a good linear relationship in the concentration range of 4.0~80μg/mL(r=0.9999),with an average recovery of 101.59%and RSD of 1.87%(n=6).The method developed in this paper was simple,accurate and reproducible.It can be used for the determination of total flavonoids and mangiferin in mango flesh.
作者
刘常凯
杨黎
何林飞
LIU Chang-kai;YANG Li;HE Lin-fei(Guangxi-Asean Food and Drug Safety Inspection and Testing Center,Nanning 530021,China)
出处
《湖北农业科学》
2020年第8期138-140,144,共4页
Hubei Agricultural Sciences
关键词
总黄酮
芒果苷
紫外-分光光度计
液相色谱法
total flavonoids
mangiferin
uv-spectrophotometer
liquid chromatography
