摘要
对红螯螯虾转录组测序数据进行分析,并开发引物通过PCR扩增和毛细管电泳检测,进行了引物多态性和通用性分析。从67369条Unigenes中共检测到22727个简单重复序列(SSR)位点,并对包含SSR序列的Unigenes进行功能注释。结果表明,红螯螯虾转录组中微卫星序列的类型较为丰富,其中二核苷酸和三核苷酸重复类型出现频率较多,分别占总SSR出现频率的52.67%和44.25%;四核苷酸、五核苷酸和六核苷酸重复类型出现频率较少,分别占总SSR出现频率的2.62%、0.23%和0.24%。SSR重复类型共有68种,其重复次数的范围为5~84次。筛选出SSR引物5对,对浙江本地及台湾2个群体的60个样品进行遗传多样性分析,每对引物平均产生5.6个多态性片段。本研究结果为深入开发红螯螯虾功能性SSR标记奠定了基础,也为开展红螯螯虾分子标记辅助选育提供支持。
The transcriptome sequencing data of the red claw crayfish(Cherax quadricarinatus)was analyzed,and the primers were developed by PCR amplification and capillary electrophoresis,the primer polymorphism and versatility analysis were carried out.22,727 simple repeat(SSR)sites were detected from 67,369 Unigenes,and functional annotations were performed on Unigenes contain⁃ing SSR sequences.The results show that,the microsatellite sequences in the transcriptome of the red claw crayfish are abundant,and the dinucleotide and trinucleotide repeat types appear more frequently,accounting for 52.67%and 44.25%of the total SSR frequen⁃cy,respectively;the tetranucleotide pentanucleotide and hexanucleotide repeat types appeared less frequently,accounting for 2.62%、0.23%and 0.24%of the total SSR frequency,respectively.There are 68 SSR repeat types,and the number of repetitions ranges from 5 to 84.5 pairs of SSR primers were screened,and 60 samples from Zhejiang and Taiwan were analyzed for genetic diversi⁃ty,the average polymorphic fragment per every prime was 5.6.The results of this study lay the foundation for the in-depth develop⁃ment of functional SSR markers of red claw crayfish,and also support the molecular marker-assisted breeding of red claw crayfish.
作者
李喜莲
郭建林
李倩
施伟达
黄振远
顾志敏
LI Xi-lian;GUO Jian-lin;LI Qian;SHI Wei-da;HUANG Zhen-yuan;GU Zhi-min(Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture/Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province/Zhejiang Institute of Freshwater Fisheries,Huzhou 313001,Zhejiang,China)
出处
《湖北农业科学》
2020年第7期207-211,共5页
Hubei Agricultural Sciences
基金
湖州市公益性应用研究项目(2016GY14)。
关键词
红螯螯虾
转录组
SSR
位点信息
Cherax quadricarinatus
transcriptome
SSR
information of loci
