摘要
本文旨在研究间接共培养条件下血管内皮细胞分泌或代谢产生的物质对于肝癌细胞上皮-间质转化(EMT)的影响。体外培养人肝癌细胞系QGY-7703,并与人脐静脉内皮细胞(HUVEC)条件培养基进行共培养;倒置相差显微镜观察肝癌细胞形态学的变化;划痕实验分析肝癌细胞迁移能力的变化;Western blot及免疫荧光实验检测条件培养基对肝癌细胞EMT相关蛋白表达及分布的影响。结果表明,共培养后,QGY-7703细胞逐渐由多边形变为纺锤形,迁移能力显著提高,EMT相关标志物的表达及分布均出现时序性变化。研究结果证实,间接共培养条件下,血管内皮细胞可诱导肝癌细胞发生EMT。
This study aims to investigate the effect of substances secreted or metabolized by vascular endothelial cells on epithelial-mesenchymal transition(EMT)of hepatocellular carcinoma cells under indirect co-culture condition.Human hepatocellular carcinoma cell line QGY-7703 was cultured in vitro,and then was co-cultured with conditioned medium of human umbilical vein endothelial cells(HUVEC).The morphological changes of QGY-7703 cells were observed by inverted phase contrast microscopy.The migration ability of QGY-7703 cells was analyzed by scratch-wound assays.The effect of conditioned medium on the expression and distribution of EMT related proteins was detected by Western blot and immunofluorescence assays,respectively.The results showed that the QGY-7703 cells gradually changed from polygonal to spindle shape,the migration ability promoted significantly,and both the expression and distribution of EMT related marker changed in a time-dependent manner after co-culturing.The results confirm that vascular endothelial cells can induce EMT in hepatocellular carcinoma cells under indirect co-culture condition.
作者
许博闻
何佳
高文博
苏冠月
刘肖珩
沈阳
XU Bowen;HE Jia;GAO Wenbo;SU Guanyue;LIU Xiaoheng;SHEN Yang(Institute of Biomedical Engineering,West China School of Basic Medical Sciences and Forensic Medicine,Sichuan University,Chengdu 610041,P.R.China)
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2020年第3期442-449,共8页
Journal of Biomedical Engineering
基金
国家自然科学基金资助项目(31870939,31570948)。
关键词
肿瘤微环境
肿瘤血管新生
上皮-间质转化
间接共培养
tumor microenvironment
tumor angiogenesis
epithelial-mesenchymal transition
indirect co-culture
