摘要
目的探讨血清维生素A、D以及胎盘组织中维生素D受体水平与子痫前期发病的相关性。方法前瞻性研究,选取2017年1月至2019年1月在泰安市中心医院定期产检伴有子痫前期孕妇112例作为研究组,另选择同期40例正常妊娠分娩孕妇作为对照组。根据子痫前期严重程度将研究组分为轻度子痫前期(63例)和重度子痫前期(49例),根据分娩结果将研究组分为分娩正常(23例)和分娩异常(89例)。使用全自动生活仪检测两组孕妇血清中1,25二羟基维生素D 3水平,使用高效液相色谱仪检测两组孕妇血清中维生素A水平,采用免疫组化法检测两组孕妇胎盘组织中维生素D受体水平。结果对照组孕妇血清中维生素A、1,25二羟基维生素D 3水平分别为(0.41±0.10)mg/L、(24.14±2.56)mg/L,胎盘组织中维生素D受体表达阴性、弱阳性和强阳性的百分比分别为0、62.50%、37.50%;研究组孕妇血清中维生素A、1,25二羟基维生素D 3水平分别为(0.30±0.09)mg/L、(17.32±1.67)mg/L,胎盘组织中维生素D受体表达阴性、弱阳性和强阳性的百分比分别为16.07%、51.79%、42.14%;对照组孕妇血清中维生素A、1,25二羟基维生素D 3水平及胎盘组织中维生素D受体表达强度均对照组孕妇血清中维生素A、1,25二羟基维生素D 3水平及胎盘组织中维生素D受体表达强度均显著低于研究组,差异有统计学意义(P<0.05);轻度子痫前期孕妇血清中维生素A、1,25二羟基维生素D 3水平分别为(0.35±0.05)mg/L、(19.14±0.89)mg/L,胎盘组织中维生素D受体表达阴性、弱阳性和强阳性的百分比分别为7.94%、52.38%、39.68%;重度子痫前期孕妇血清中维生素A、1,25二羟基维生素D 3水平分别为(0.24±0.04)mg/L、(15.32±0.66)mg/L,胎盘组织中维生素D受体表达阴性、弱阳性和强阳性的百分比分别为26.53%、51.02%、22.45%;轻度子痫前期孕妇血清中维生素A、1,25二羟基维生素D 3水平及胎盘组织中维生素D受�
Objective To explore the relationship between the levels of Vitamin A,Vitamin D in serum and Vitamin D receptor in placenta and the pathogenesis of preeclampsia.Methods In this prospective study,112 pregnant women with pre-eclampsia who had routine pregnancy check-up in our hospital from January 2017 to January 2019were selected as the study group,and 40 normal pregnant women during the same period were selected as the control group.According to the severity of preeclampsia,the study group was divided into mild preeclampsia(63 cases)and the severe preeclampsia(49 cases).According to the delivery condition,the study group was divided into normal delivery(23 cases)and abnormal delivery(89 cases).The level of 1,25 dihydroxyvitamin D 3 in serum was detected by the automatic biochemical analyzer,the level of vitamin A in serum was detected by the high performance liquid chromatography,and the vitamin D receptor in placenta was detected by immunohistochemistry.Results The serum levels of vitamin A,1,25 dihydroxyvitamin D 3 in the control group were(0.41±0.10)mg/L and(24.14±2.56)mg/L respectively,and the negative,weak positive and strong positive rates of vitamin D receptor in placenta were 0.00%,62.50%and 37.50%respectively.The serum levels of vitamin A,1,25 dihydroxyvitamin D 3 in the study group were(0.30±0.09)mg/L and(17.32±1.67)mg/L respectively)mg/L,and the negative,weak positive and strong positive rates of vitamin D receptor in placenta were 16.07%,51.79%and 42.14%respectively.The levels of Vitamin A,1,25 dihydroxyvitamin D 3 in serum and the expression of Vitamin D receptor in placenta in the control group were significantly lower than those in the study group(P<0.05).The levels of vitamin A,1,25 dihydroxyvitamin D 3 in the serum of mild preeclampsia were(0.35±0.05)mg/L and(19.14±0.89)mg/L,and the negative,weak positive and strong positive rates of vitamin D receptor in the placenta were 7.94%,52.38%and 39.68%respectively.The levels of vitamin A,1,25 dihydroxyvitamin D 3 in the serum of severe preeclampsi
作者
黄玲
赵洁
杜鹃
张军
董雪
李允广
邓玉玲
HUANG Ling;ZHAO Jie;DU Juan(Department of Obstetrics,Tai'an Central Hospital,Tai'an Shandong 271000,China;Department of Obstetrics,Ji'nan Second Maternal and Child Health Hospital,Ji'nan Shandong 271100,China)
出处
《临床和实验医学杂志》
2020年第11期1188-1191,共4页
Journal of Clinical and Experimental Medicine
基金
国家卫生计生委医药卫生科技发展项目(编号:W2015CAE085)。
