摘要
目的筛选乌饭树果乙醇提取物中抗氧化活性最强的极性分部。方法采用石油醚、乙酸乙酯、氯仿、正丁醇和水等5种不同极性溶剂对乌饭树果乙醇提取物进行进一步梯度萃取,并分析比较其总抗氧化能力(total antioxidant capacity,T_(AC))、抗脂质过氧化能力及对羟基自由基OH·、超氧阴离子自由基O_2^-·的清除效果。结果乌饭树果乙醇提取物的5个不同极性分部均具有一定的抗氧化活性,但其活性大小差异极显著(P<0.01),T_(AC)大小依次为:乙酸乙酯部>氯仿部>正丁醇部>石油醚部>水部。同时,在OH·、O_2^-·自由基清除能力和抗脂质过氧化能力上,乙酸乙酯部均优于氯仿部和正丁醇部,且呈明显的量效关系。结论乌饭树果乙醇提取物的乙酸乙酯部具有最强的抗氧化活性,且对OH·、O_2^-·的清除能力及抗脂质过氧化能力均强于L-抗坏血酸及其它部位,可作为天然抗氧化剂的潜在有效来源。
Objective To screen the strongest antioxidant activities of polarity fraction of ethanol extract obtained from Vaccinium bracteatum Thunb.fruits.Methods Totally 5 different polarity solvents(petroleum ether,ethyl acetate,chloroform,n-butanol and water) were used to furtherly fractionate ethanol extracts from Vaccinium bracteatum Thunb.fruits.Moreover,the total antioxidant capacity(TAC),anti-lipid peroxidation and scavenging effect of 5 kinds of polarity fractions on hydroxyl radical(OH·) and superoxide radical(O2-·) were studied and compared.Results A total of 5 polarity fractions of ethanol extract obtained from Vaccinium bracteatum Thunb.fruits were showed antioxidant activity while exhibited significant differences(P<0.01),among which the ethyl acetate fraction had the strongest TAC,following by the chloroform,n-butanol fractions and the petroleum ether,the water fraction was the weakest.Furthermore,the ethyl acetate fraction had better scavenging capacities of OH·,O2-·and anti-lipid peroxidation than n-butanol fraction and chloroform fraction,and also showed a significant dose-response relationship.Conclusion The ethyl acetate fraction exhibited the strongest of antioxidant capacity,was superior to L-ascorbic acid and other fractions on the scavenging effects of OH·,O2-· and anti-lipid peroxidation,which could be used as a potential effective source of natural antioxidants.
作者
康彬彬
刘龙燕
郭溪远
王祥
张玲艳
陈团伟
KANG Bin-Bin;LIU Long-Yan;GUO Xi-Yuan;WANG Xiang;ZHANG Ling-Yan;CHEN Tuan-Wei(Fujian Vocational College of Bioengineerings Fuzhou 350007,China;College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《食品安全质量检测学报》
CAS
2020年第10期3124-3129,共6页
Journal of Food Safety and Quality
基金
福建省中青年教师教育科研项目(JAT160763)
福建农林大学创新性项目(CXZX2016093)。
关键词
乌饭树果
乙醇提取物
极性分部
抗氧化活性
Vaccinium bracteatum Thunb.fruits
ethanol extract
polar fractions
antioxidant activity
