摘要
目的建立一种用猪角膜进行消毒产品急性眼刺激性体外检测的方法。方法采用乙醇和咪唑2种阳性物作为质控标准品,在猪角膜体系上进行体外试验条件的探索。结果经液体和固体2种消毒剂验证得出,乙醇暴露10 min、孵育80 min和咪唑暴露3 h、孵育70 min的条件下,猪角膜体外刺激评分在牛角膜混浊与通透性(bovine corneal opacity and permeability,BCOP)试验相应的阳性物标准范围内,该试验系统成立。结论用猪眼代替牛眼初步建立了一种消毒剂急性眼刺激性试验方法,可为消毒产品安全性检测提供参考。
Objective To establish an in vitro test method for acute eye irritation of disinfection products by using porcine cornea.Methods In vitro detection conditions on porcine corneal system were investigated and optimizated,which used ethanol and imidazole as positive control.Results It was confirmed by liquid and solid disinfectants that after the exposure of ethanol for 10 min and incubation for 80 min,or the imidazole exposure for 3 h and incubation for 70 min,the in vitro stimulation score was within the standard range of bovine corneal opacity and permeability(BCOP)corresponding positives,and the test system was established.Conclusion A method for detecting acute eye irritation of disinfectants is initially established by using pig eyes instead of bovine eyes,which can provide a reference for the safety evaluation of disinfection products.
作者
李敏
李光先
李培宁
陈梓灵
刘颖
朱素珍
庞增雄
刘冬虹
刘香梅
LI Min;LI Guangxian;LI Peining;CHEN Ziling;LIU Ying;ZHU Suzhen;PANG Zengxiong;LIU Donghong;LIU Xiangmei(Guangzhou Quality Supervision and Testing Institute,Guangzhou,511447,China)
出处
《实验动物与比较医学》
CAS
2020年第3期248-251,共4页
Laboratory Animal and Comparative Medicine
基金
广东省质量监督管理局科技项目(2018PZ07)。
关键词
消毒产品
猪角膜
牛角膜
眼刺激
Disinfection products
Porcine cornea
Bovine cornea
Eye irritation
