摘要
目的探讨炎症性肠病(IBD)患者小肠上皮细胞中维生素D受体(VDR)的表达水平与自噬流受损之间的相关性。方法回顾性收集41例IBD患者和13例正常对照者的小肠黏膜组织,分别设为IBD组和正常对照组。采用免疫组化检测两组小肠上皮细胞自噬流相关标志物(LC3、p62)和VDR蛋白的表达。统计分析VDR和LC3以及p62的相关性。结果与正常对照组相比,IBD患者小肠上皮细胞中VDR表达显著下降,而LC3和p62的表达增加(均P<0.0001),提示自噬流受损。VDR的表达与LC3(r=-0.59,P<0.01)和p62(r=-0.62,P<0.01)的表达呈负相关。结论IBD患者中VDR表达量的减少可能与自噬流受损有关。
Objective To explore the correlation of vitamin D receptor(VDR)expression with autophagic flow injury of small intestinal epithelial cells in patients with inflammatory bowel disease(IBD).Methods The small intestinal mucosa from 41 patients with IBD and 13 normal control subjects was collected,which were set as IBD group and normal control group respectively.The expressions of VDR and autophagic flow-associated proteins LC3 and p62 of small intestinal epithelial cells were detected by immunohistochemistry.The correlations between VDR expression and LC3 or p62 were analyzed by Spearman method.Results Compared with normal control group,the expression of VDR decreased obviously in IBD group,while the expressions of LC3 and p62 increased significantly(all P<0.0001),which indicated the injured autophagic flow.The expression of VDR was negatively correlated with expression of LC3(r=-0.59,P<0.01)and p62(r=-0.62,P<0.01).Conclusion The lower expression of VDR may be correlated with the autophagic flow injury in IBD patients.
作者
王玫
何伟
陈建平
Wang Mei;He Wei;Chen Jianping(Department of Gastroenterology,The First People′s Hospital of Changzhou,Changzhou 213003,China)
出处
《中华炎性肠病杂志（中英文）》
2020年第2期139-141,共3页
Chinese Journal of Inflammatory Bowel Diseases
关键词
炎症性肠病
维生素D受体
自噬
自噬流
Inflammatory bowel disease
Vitamin D receptor
Autophagy
Autophagic flow
