摘要
为研究碱性体系下美拉德反应产物中的关键香味成分,建立了一种碱性体系的美拉德反应产物制备方法,并通过GC-MS联用技术分析了4种糖类和20种氨基酸美拉德反应产物中的化学成分.选择美拉德反应产物中的氮氧硫杂环类化合物、芳香族类化合物和烯酮(醛)类化合物关键香味成分作为研究对象,获得了多种美拉德反应产物中关键香味成分种类及质量分数的分布特点.结果表明:①在众多关键香味成分中,含氮杂环化合物种类多且质量分数大,存在于绝大多数美拉德反应产物中;含氧杂环化合物种类和质量分数较少,主要存在于葡萄糖和果糖美拉德反应的产物中;含硫杂环化合物种类和质量分数较少,只存在于半胱氨酸的美拉德反应产物中;芳香族类化合物和烯酮(醛)类化合物种类和质量分数较少,只在一些特定氨基酸的美拉德反应产物中存在.②糖类美拉德反应产物关键香味成分的总质量为果糖>>木糖>葡萄糖>脱氧核糖.③不同氨基酸美拉德反应产物中关键香味成分总质量差别明显,其中总质量较高对应的氨基酸有亮氨酸、赖氨酸、苯丙氨酸和色氨酸.
A method for preparing Maillard products under basic conditions was developed.The Maillard reaction products from 4 sugars and 20 amino acids were analyzed with GC/MS.Nitrogen-,oxygen-,sulfur-containing heterocyclic compounds,aromatic compounds and ketene(enal)compounds were selected as key aroma components,and the types and contents were determined.The results showed that:1)Among the key aroma components,nitrogen-containing heterocyclic compounds were higher in almost all the Maillard reactions.The types and amounts of oxygen-containing heterocyclic compounds were lower,and mainly formed by glucose or fructose.Sulfur-containing heterocyclic compounds were less in the type and content,and formed by cysteine.The types and contents of aromatic compounds and ketene(enal)compounds were less,and formed by some specific amino acids.2)In the order of the total contents of key aroma components by sugars were:fructose>>xylose>glucose>deoxyribose.3)The total contents of key aroma components from the different amino acids were different,and the contents from leucine,lysine,phenylalanine and tryptophan were higher.
作者
朱龙杰
张华
吴洋
陈晶波
曹毅
廖惠云
王瑞
朱怀远
沈晓晨
毛淑蕊
ZHU Longjie;ZHANG Hua;WU Yang;CHEN Jingbo;CAO Yi;LIAO Huiyun;WANG Rui;ZHU Huaiyuan;SHEN Xiaochen;MAO Shurui(Technology Center,China Tobacco Jiangsu Industrial Co.,Ltd.,Nanjing 210019,China)
出处
《烟草科技》
EI
CAS
CSCD
北大核心
2020年第6期41-47,80,共8页
Tobacco Science & Technology
基金
江苏中烟工业有限责任公司科技项目“美拉德反应产物在调香技术中的应用研究”(2018-03)。
关键词
美拉德反应
反应产物
碱性体系
关键香味成分
糖类
氨基酸
杂环化合物
Maillard reaction
Reaction product
Alkaline system
Key aroma component
Sugar
Amino acid
Heterocyclic compound
