摘要
目的描述炎症性肠病患者的粪便微生物群落组成,找出与炎症性肠病相关的菌群,为疾病的治疗和诊断提供新思路。方法收集克罗恩病(n=20)、溃疡性结肠炎(n=10)和健康对照组(n=30)的粪便样本,利用16S rRNA测序法,测定粪便样品中微生物群落组成。结果炎症性肠病患者肠道菌群与正常对照组有显著差异。炎症性肠病患者变形菌门、Epsilonbacteraeota、酸杆菌门、志贺氏菌丰度显著升高;普氏菌属_9、巨单胞菌属、Lachnoclostridium丰度显著降低。克罗恩病组Lachnoclostridium丰度显著高于溃疡性结肠炎组,酸杆菌门、普氏菌属_9丰度显著低于溃疡性结肠炎组。厚壁菌门、拟杆菌门丰度在炎症性肠病患者中降低,但差异无统计学意义(P>0.05)。结论炎症性肠病患者肠道微生物失调,IBD患者肠道菌群中致病菌增多(主要为变形菌门和大肠埃希菌志贺菌)。克罗恩病组和溃疡性结肠炎组的差异菌群可能和疾病相关。
Objective To describe the composition of fecal microbiota in patients with inflammatory bowel disease,identify the bacteria related to inflammatory bowel disease,and to provide new ideas for the treatment and diagnosis of the disease.Methods Fecal samples were obtained from patients with Crohn’s disease(CD,n=20),ulcerative colitis(UC,n=10)and healthy controls(n=30).A 16S rRNA sequencing approach was applied to determine the compositions of microbial communities in fecal samples.Results The microbial structure of patients with inflammatory bowel disease was significantly different from that of the control group.Abundance of Proteobacteria,Epsilonbacteraeota,Acidobacteria,Escherichia-Shigella were significantly higher in IBD patients relative to healthy controls,whereas significantly lower abundances were observed for Prevotella_9,Megamonas and Lachnoclostridium.The abundance of Lachnoclostridium in Crohn′s disease was significantly higher than that in ulcerative colitis,while Acidobacteria and Prevotella_9 was significantly lower than that in ulcerative colitis.The abundance of Firmicutes and Bacteroidetes were decreased in disease cohorts,but the difference was not statistically significant(P>0.05).Conclusions Intestinal microbial disorders in patients with IBD,and increased pathogenic bacteria(mainly proteobacteria and shigella Escherichia coli)in the intestinal microflora of IBD patients.The difference in microbiota between Crohn′s disease and ulcerative colitis may be related to the disease.
作者
田丽
黄颉刚
梁浩
叶力
崔萍
钟晓丹
张毓
周杰
陈晖
Tian li;Huang Jiegang;Liang Hao;Ye Li;Cui Ping;Zhong Xiaodan;Zhang Yu;Zhou Jie;Chen Hui(The First Affiliated Hospital of Guangxi Medical University,Nanning 530000,China;Guangxi Key Laboratory of AIDS Prevention and Treatment,School of Public Health,Guangxi Medical University,Nanning 530000,China)
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2020年第2期180-185,共6页
Chinese Journal of Experimental and Clinical Virology
基金
国家自然科学基金(81660334)
广西自然科学基金(2017JJA10216,2018JJA140886)
"十三五"国家科技专项课题(2018ZX10101002-001-006)。
