摘要
目的观察穴位贴敷对氧嗪酸钾致高尿酸血症模型大鼠血尿酸(SUA)的干预作用,探讨穴位贴敷对肾脏尿酸排泄的作用机制。方法将32只雄性SD大鼠,随机分为空白组、模型组、假贴敷组、穴位贴敷组,每组8只。实验采用氧嗪酸钾灌胃制备高尿酸血症模型,穴位贴敷组采用降酸贴贴敷于肝脾肾的俞募穴,连续贴5 d为1个疗程,共干预3个疗程;假贴敷组使用凡士林制作的假贴剂,其贴敷方法同穴位贴敷组,空白组与模型组不施加干预处理。3个疗程后,测定SUA、血肌酐(SCr)等血尿生化指标,计算尿酸排泄指标,包括24 h尿酸排泄量(24 h UUA)、尿酸排泄分数(FEUA)、尿酸清除率(CUr),苏木精-伊红(HE)染色法观察肾脏组织病理学变化,免疫组化法测定尿酸盐阴离子转运体1(URAT1)和葡萄糖转运体9(GLUT9)的表达。结果与模型组和假贴敷组比较,穴位贴敷组SUA明显降低(P<0.01),尿酸排泄指标升高(P<0.01),URAT1和GLUT9的表达显著降低(P<0.05),肾脏组织病理变化减轻。结论穴位贴敷可显著降低HUA模型大鼠SUA水平,其机制可能为抑制大鼠肾脏URAT1和GLUT9的蛋白表达,减少尿酸的重吸收,促进尿酸的排泄,并减少高尿酸堆积于肾脏,降低对肾脏的损害。
Objective:To observe the effect of acupoint application on serum uric acid(SUA)in hyperuricemia rats induced by potassium oxyzinate,and to explore the mechanism of acupoint application on renal uric acid excretion.Methods:32 male SD rats were randomly divided into the blank group,the model group,false acupoint application group and acupoint application group,8 rats in each.Hyperuricemia model was established by intragastric administration of potassium oxyzinate.Acupoint application group used acid-lowering patches to the Shu-Mu acupoints of liver,spleen,kidney,continuous application for 5 days as a course of treatment,3 courses in total.False acupoint application group used Vaseline patches in the same way as acupoint application group;the blank group and the model group did not apply any treatment.After three courses of treatment,SUA,SCr and other biochemical indexes of hematuria were measured.The excretion index of uric acid was calculated,including 24 hUUA,FEUA and CUr.He staining was used to observe renal histopathological changes.The expression of URAT1 and GLUT9 were detected by immunohistochemistry.Results:Compared with the model group and the false acupoint application group,SUA decreased(P<0.01);uric acid excretion index increased(P<0.01);the expression of URAT1 and GLUT9 was significantly decreased(P<0.05),and the pathological changes of kidney tissue were slight in acupoint application group.Conclusion:Acupoint application can significantly reduce the level of SUA,whose mechanism may be to inhibit the protein expression of URAT1 and GLUT9,reduce the reabsorption of uric acid,promote the excretion of uric acid,reduce the accumulation of high uric acid in the kidney and reduce the damage to the kidney.
作者
何晓茜
唐雪青
刘佳男
吴雪梅
睢明河
He Xiaoqian;Tang Xueqing;Liu Jianan;Wu Xuemei;Sui Minghe(Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《中国中医急症》
2020年第6期1040-1044,共5页
Journal of Emergency in Traditional Chinese Medicine
基金
北京中医药大学创新创业自主课题(2019-JYB-XSCXCY-03)
北京中医药大学自主选题项目(2015-JYB-XS127)。
关键词
高尿酸血症
穴位贴敷
尿酸排泄
尿酸转运蛋白
俞募配穴
大鼠
Hyperuricemia
Acupoint application
Uric acid excretion
Uric acid transporter
Shu-Mu acupoints
Rats
