摘要
目的:探讨肿瘤坏死因子受体相关因子-6(TRAF-6)、白介素1受体关联激酶-1(IRAK-1)、嗜中性白细胞碱性磷酸酶-3(NALP3)等3种炎症因子失调在痛风性关节炎患者体内的作用。方法:选取本院收治的105例痛风性关节炎患者(急性发作期患者47例,缓解期患者58例,即A、B组)进行回顾性实验,同时选健康志愿者61例进行对照,即C组,3组纳入时间均为2017年5月~2018年5月,所有受试者TRAF-6、IRAK-1和NALP3检测均采用实时荧光定量发法(RT-PCR)完成,并比较这3种炎性因子与痛风性关节炎的相关性。结果:(1)A、B组治疗后的ESR、BUA及总补体均高于C组,其中A组上述3项指标均高于B组(P<0.05),而CRP均低于C组,且A组上述两项指标均低于B组(P<0.05)。(2)A、B组治疗前TRAF-6 mRNA相对表达量比较无明显差异(P<0.05),且低于C组(P<0.05);A、B组治疗后的上述指标均有所提升,但A组依旧低于B组(P<0.05),且A组提升幅度同样低于C组(P<0.05),而B组提升幅度较C组无明显差异(P<0.05)。(3)A、B组治疗前的IRAK-1mRNA相对表达量相比无显著差异(P>0.05),但均低于C组(P<0.05),A、B组治疗后的IRAK-1mRNA相对表达量均有所提升,A组明显低于C组(P<0.05),B组较C组则无明显差异(P>0.05)。(4)A、B组治疗前的NALP-3 mRNA相对表达量相比无明显差异(P>0.05),且高于C组(P<0.05);A组治疗后的NALP-3 mRNA相对表达量下降均不明显(P<0.05),而B组治疗后则明显下降,与A、C组相比均有显著差异(P<0.05)。(5)TRAF-6与ESR、CRP及总补体均无相关性(P>0.05);IRAK-1与CRP、BUA及总补体呈负相关(P<0.05);NALP-3与ESR、CRP呈正相关(P<0.05)。结论:在痛风性关节炎患者的患病过程中,TRAF-6、IRAK-1及NALP-3均呈异常表达状态,是促进患者病情发生、发展和转归的重要参与者,应采取措施进行干预。
Objective:To investigate the role of tumor necrosis factor receptor related factor 6(TRAF-6),IL 1 receptor associated kinase 1(IRAK-1)and neutrophil alkaline phosphatase 3(NALP3)in patients with gouty arthritis.Methods:Totally 105 patients with gouty arthritis(47 patients with high activityof group A,57 patients with low activity of groupB)were selected for retrospective experiment,and 61 healthy volunteers were selected as control,group C for control.The three groups were included from May 2017 to may 2018.All subjects'TRAF-6,IRAK-1 and NALP-3 were detected by real-time fluorescence quantitative method(RT-PCR)was performed and the correlation between these three inflammatory factors and gouty arthritis was compared.Result:1)ESR,BUA and total complement in group A and B were higher than those in group C,and the three indexes detected in group A were higher than those in group B(P<0.05),while CRP were lower than those in group C,and the two indexes in group A were lower than those in group B(P<0.05);2)there was no significant difference in the relative expression of TRAF-6 mRNA between group A and group B before treatment(P>0.05),and it was significantly lower than that of group C(P<0.05);after treatment,the above indexes in group A and group B were all improved,but the indexes in group A were still lower than those in group B(P<0.05),and the elevation range in group A was also lower than that in group C(P<0.05),while the elevation range in group B was not significantly different from that in group C(P>0.05);3)there was no significant difference in the relative expression of IRAK-1 mRNA between group A and group B before treatment(P>0.05),but it was lower than that of group C(P<0.05).The relative expression of IRAK-1 mRNA in group A and group B after treatment was increased,which was significantly lower than that in group C(P<0.05),but there was no significant difference between group B and group C(P>0.05);4)there was no significant difference in the relative expression of NALP-3 mRNA between group A and group B b
作者
谢敏
范晓利
赵一璟
王昆
XIE Min;FAN Xiao-li;ZHAO Yi-jing;WANG Kun(Department of Endocrinology, Jiangning Hospital, Nanjing Jiangsu, 211100, China)
出处
《海南医学院学报》
CAS
2020年第12期925-931,937,共8页
Journal of Hainan Medical University
基金
南京医科大学科技发展基金一般项目(NMUB2018163)。
