摘要
目的通过构建鼠巨细胞病毒(MCMV)腹腔感染小鼠模型,探讨鼠MCMV与血管平滑肌增殖重塑的关系。方法通过MCMV腹腔感染7月龄C57BL/6J小鼠模型,将C57BL/6J小鼠分为对照组与实验组(MCMV感染组),每组10只;在9月龄及12月龄时使用颈动脉测压比较两组血压变化情况,并分别留取胸主动脉组织切片进行HE、Masson染色,观察小鼠主动脉重塑及纤维化程度,利用image pro-plus软件计算主动脉胶原面积占全部面积比值,通过Western Blot检测血管重塑蛋白α-平滑肌肌动蛋白(α-SMA)、骨桥蛋白(OPN)、细胞增殖核抗原(PCNA)的表达。结果 MCMV感染组小鼠血压升高,胸主动脉中膜增厚增加,胸主动脉胶原面积百分比增加,血管重塑蛋白由收缩表型向合成表型转化。结论 MCMV感染可引起小鼠血压升高,并导致胸主动脉重塑。
Objective In order to explore the relationship between the proliferation and remodeling of vascular smooth muscle,a mouse model of intraperitoneal infection with mouse cytomegalovirus(MCMV)was established.Methods C57BL/6J mice were divided into control group and experimental group(MCMV infection group)by intraperitoneal infection of MCMV.The changes of blood pressure in the two groups were compared by carotid manometry at the age of 9 and 12 months,and the sections of thoracic aorta were taken for he and Masson staining to observe the degree of aortic remodeling and fibrosis of mice The ratio of collagen area to total area was calculated by e Pro Plus software.The expression ofα-SMA,OPN and PCNA were detected by Western blot.Results In the MCMV infected mice,the blood pressure increased,the thickness of the middle membrane of the thoracic aorta increased,the percentage of the collagen area of the thoracic aorta increased,and the vascular remodeling protein transformed from contraction phenotype to synthesis phenotype.Conclusion MCMV infection can increase blood pressure and lead to remodeling of thoracic aorta in mice.
作者
黄桢
石运忠
唐娜
陈璋玥
徐浩文
汤利
常红霞
李凯芳
何芳
HUANG Zhen;SHI Yunzhong;TANG Na;CHEN Zhangyue;XU Haowen;TANG Li;CHANG Hongxia;LI Kaifang;HE Fang(School of Medicine,Shihezi University,Shihezi,Xinjiang 832000,China)
出处
《石河子大学学报（自然科学版）》
CAS
北大核心
2020年第3期347-352,共6页
Journal of Shihezi University(Natural Science)
基金
国家自然科学基金-地区科学基金项目(3176070019)
石河子大学大学生创新创业训练计划项目(201910759024)
石河子大学自主资助支持校级科研立项项目(ZZZC201921A)。
关键词
MCMV
高血压
血管重塑
MCMV
hypertension
vascular remodeling
