摘要
目的探讨蛋白质精氨酸甲基转移酶5(Protein arginine methyltransferase 5,PRMT5)对人肝癌细胞生物学行为的影响。方法应用免疫印迹法(Western blot)检测正常肝细胞LO2、肝癌细胞HepG2及Huh7中PRMT5的表达水平,应用siRNA技术将PRMT5基因敲减,流式细胞仪检测肝癌细胞HepG2及Huh7的凋亡情况。应用Western blot检测蛋白激酶B(Protein kinase B,AKT)及磷酸化蛋白激酶B(Phosphorylated protein kinase B,P-AKT)的表达水平。结果与正常肝细胞LO2相比,PRMT5在肝癌细胞HepG2及Huh7中高表达(P<0.05)。抑制PRMT5的表达可以促进肝癌细胞凋亡。将PRMT5基因敲减后,肝癌细胞系中P-AKT的表达量降低(P<0.01)。结论PRMT5可能通过调控肝细胞肝癌(Hepatocellular Carcinoma,HCC)中AKT通路从而促进肝癌细胞凋亡。因此,PRMT5可能是肝癌的潜在生物标志物和有希望的治疗靶标。
Objective To investigate the effect of protein arginine methyltransferase 5(PRMT5)on the biological behavior of human hepatocellular carcinoma cells.Methods The expression of PRMT5 was detected with Western blot in normal liver cell line LO2 and hepatocellular carcinoma cell lines HepG2 and Huh7 cells.siRNA technology was used to knockdown the expression of PRMT5 and flow cytometry was used to detect the apoptosis of HepG2 and Huh7 cells.The protein expression of protein kinase B(AKT)and phosphorylated protein kinase B(P-AKT)was detected by Western blot.Results Compared with LO2 cells,PRMT5 was highly expressed in hepatocellular carcinoma cell lines HepG2 and Huh7(P<0.05).Inhibition of PRMT5 expression could promote apoptosis of HCC cells.The expression of P-AKT was decreased when knocked-down the expression of PRMT5 gene(P<0.01).Conclusion PRMT5 may promote HCC(Hepatocellular Carcinoma)cell apoptosis by regulating AKT pathway.Therefore,PRMT5 may be a potential biomarker and promising therapeutic target for hepatocellular carcinoma.
作者
周健
陈雪健
王伟
李宁
孙振宇
徐力善
ZHOU Jian;CHEN Xuejian;WANG Wei;LI Ning;SUN Zhenyu;XU Lishan(Department of Oncological and Hepatobiliary Surgery,The Fourth Affiliated Hospital of Harbin Medical University,Harbin 150001,China;Department of Anorectal Surgery,The First Hospital of Ningbo City)
出处
《实用肿瘤学杂志》
CAS
2020年第3期208-213,共6页
Practical Oncology Journal
基金
黑龙江省科研计划项目(编号:201710)
哈尔滨医科大学附属第四医院火炬计划(编号:HYDSYHJ201903)。
