摘要
为研究阿拉山口口岸小型兽类巴尔通体感染情况,对捕获的75只小型兽类进行形态学鉴定后,提取肝脏和脾脏核酸扩增巴尔通体Bartonella枸橼酸合酶基因(gltA),通过阳性序列的比对进行巴尔通体分子鉴定和序列分析。结果显示,阿拉山口口岸小型兽类巴尔通体感染率为9.33%(7/75),分别为大沙鼠3只、小家鼠2只、红尾沙鼠1只、小林姬鼠1只。经测序,7份阳性样本获得的巴尔通体分属于伊丽莎白巴尔通体、格拉汉姆巴尔通体、抚远巴尔通体和泰勒巴尔通体4种,其中3个阳性为伊丽莎白巴尔通体,各鼠种巴尔通体感染率无显著性差异。伊丽莎白巴尔通体和格拉汉姆巴尔通体为鼠传人类致病性巴尔通体,应通过灭鼠、灭蚤以降低巴尔通体自然宿主或传播媒介的密度,防止巴尔通体在国境口岸的传入传出。
In order to study the infection of Bartonella in small mammals at Alashankou Port, the nucleic acids of liver and spleen were extracted and the Bartonella citrate synthase gene(gltA) was amplified after morphological identification of 75 small mammals. The molecular identification and sequence analysis of Bartonella were carried out by comparing the positive sequences. The results showed that the infection rate of Bartonella was 9.33%(7/75), including 3 Rhombomys opimus, 2 Mus musculus, 1 Meriones erythrourus, 1 Apodemus sylvaticus. Seven strains of Bartonella belonged to 4 species, B. elizabethae, B. grahamii, B. fuyuanensis and B. taylorii, of which 3 strains were B. elizabethae. SPSS analysis showed that there was no significant difference in Bartonella infection rate among different hosts. B. elizabethae and B. grahamii are the rat-borne human pathogen. Rodents control should be done to reduce the density of the natural host and vector to prevent the import and export of such pathogen, ensure the health and safety of frontier ports.
作者
王颖
王婕敏
万道正
慈颖
曹晓梅
王静
张晓龙
WANG Ying;WANG Jie-Min;WAN Dao-Zheng;CI Ying;CAO Xiao-Mei;WANG Jing;ZHANG Xiao-Long(Yantai International Travel Health Care Center,Yantai 264000,Shandong,China;Chinese Academy of Inspection and Quarantine,Beijing 100176,China;Hu’nan International Travel Health Care Center,Changsha 410100,Hu’nan,China;Pingxiang Customs,Pingxiang 532600,Guangxi,China)
出处
《寄生虫与医学昆虫学报》
CAS
2020年第1期15-19,共5页
Acta Parasitologica et Medica Entomologica Sinica
基金
国家科技重大专项项目(编号:2017ZX10303404-003-004,2017ZX10303404-003-003,2018ZX10101001-004,2017ZX10303404001004)
青岛海关科研项目(编号:QK201909)。
