摘要
目的探讨慢性阻塞性肺疾病(COPD)患者中性粒细胞氧化吞噬功能及相关受体[趋化因子C-C基序受体-1(CCR1)和Toll样受体(TLR)2、TLR4]变化的临床意义。方法建立用于检测COPD患者痰液中性粒细胞氧化吞噬功能及相关受体的流式细胞术方法。采用流式细胞术检测30例COPD患者(COPD组)、30例肺炎患者(肺炎组)和30名体检健康者(正常对照组)外周血及痰液(正常对照组除外)中性粒细胞氧化吞噬功能及其表面CCR1、TLR2和TLR4的表达水平。结果肺炎组和COPD组外周血中性粒细胞氧化吞噬功能均显著低于正常对照组(P<0.05),肺炎组与COPD组之间差异无统计学意义(P>0.05)。COPD组、肺炎组及正常对照组之间外周血中性粒细胞表面CCR1表达差异均有统计学意义(P<0.05),而TLR2、TLR4表达3组之间差异均无统计学意义(P>0.05)。COPD组痰液中性粒细胞氧化吞噬功能显著低于肺炎组(P<0.05),CCR1表达显著高于肺炎组(P<0.05),TLR2和TLR4表达2个组之间差异均无统计学意义(P>0.05)。Pearson相关分析结果显示,COPD组痰液中性粒细胞氧化吞噬阳性率与CCR1表达呈负相关(r=-0.548,P<0.01),与TLR2、TLR4表达均无相关性(r值分别为-0.206、-0.219,P>0.05)。结论COPD患者痰液中性粒细胞氧化吞噬功能显著下降,其表面CCR1表达升高。COPD患者气道中性粒细胞的氧化吞噬功能受损可能是COPD发病的机制之一。
Objective To investigate the roles of neutrophil oxidative phagocytosis and related receptors[chemokine C-C receptor-1(CCR1),Toll-like receptor(TLR)2 and TLR4]in patients with chronic obstructive pulmonary disease(COPD).Methods A flow cytometry method was established to determine neutrophil oxidative phagocytosis and related receptors in sputum samples of COPD patients.By flow cytometry,neutrophil oxidative phagocytosis in peripheral blood and sputum samples of 30 COPD patients(COPD group),30 pneumonia patients(pneumonia group)and 30 healthy subjects(healthy control group)was determined.Neutrophil oxidative phagocytosis and the expression levels of CCR1,TLR2 and TLR4 on the surface were determined.Results The neutrophil oxidative phagocytosis of peripheral blood in pneumonia group and COPD group was lower than that in healthy control group(P<0.05),and there was no statistical significance between pneumonia and COPD groups(P>0.05).There was statistical significance in the expression of CCR1 on the surface of peripheral blood neutrophils among COPD,pneumonia and healthy control groups(P<0.05),but there was no statistical significance for TLR2 and TLR4 expression levels(P>0.05).The neutrophil oxidative phagocytosis in sputum samples in COPD group was lower than that in pneumonia group(P<0.05),the expression level of CCR1 was higher than that in pneumonia group(P<0.05),and there was no statistical significance for the expression levels of TLR2 and TLR4(P>0.05).Pearson correlation analysis showed that the positive rate of neutrophil oxidative phagocytosis in sputum samples in COPD group was negatively correlated with the expression level of CCR1(r=-0.548,P<0.01),and it had no correlation with the expression levels of TLR2 and TLR4(r=-0.206 and-0.219,P>0.05).Conclusions Neutrophil oxidative phagocytosis in sputum samples of COPD patients is decreased,which can increase CCR1 expression level on the surface,suggesting that COPD patients'respiratory neutrophil oxidative phagocytosis injury may be one of the mechanisms of
作者
闫佩毅
张骥
涂焕平
张立
仇越
张锋英
胡奕雯
金姝
YAN Peiyi;ZHANG Ji;TU Huanping;ZHANG Li;QIU Yue;ZHANG Fengying;HU Yiwen;JIN Shu(Department of Clinical Laboratory,Shanghai People's Hospital of Putuo District,Shanghai 200060,China;Department of Pathology,Shanghai People's Hospital of Putuo District,Shanghai 200060,China;Department of Respiratory,Shanghai People's Hospital of Putuo District,Shanghai 200060,China;Central Laboratory,Shanghai People's Hospital of Putuo District,Shanghai 200060,China)
出处
《检验医学》
CAS
2020年第6期570-577,共8页
Laboratory Medicine
基金
上海市卫生健康委员会青年课题(20164Y0133)。
