摘要
背景与目的氟苯达唑是一种苯并咪唑类驱虫药,既往研究发现其对结肠癌、乳腺癌细胞增殖具有抑制作用。本研究旨在探讨氟苯达唑对非小细胞肺癌A549、H460细胞增殖的影响及机制。方法通过CCK-8(Cell Counting Kit-8)法检测不同浓度的氟苯达唑对A549、H460细胞活力的影响;Western blot法检测氟苯达唑处理后细胞自噬相关蛋白p62、LC3的表达水平;自噬双标腺病毒(mRFP-GFP-LC3)转染细胞,分析细胞内自噬流变化。结果氟苯达唑抑制A549、H460细胞增殖,并呈剂量依赖关系(P<0.001)。2μmol/L氟苯达唑处理A549、H460细胞24 h、48 h后p62减少,LC3 II/I比值升高(P<0.005)。mRFP-GFP-LC3转染细胞显示氟苯达唑处理组红色荧光增加,提示自噬流增强。结论氟苯达唑可以抑制A549、H460细胞增殖并促进自噬。
Background and objective Flubendazole is an anthelmintic and categorized in benzimidazole.Previous evidence indicates its suppression on proliferation of colon cancer and breast cancer cells.Our study aims to explore the effects of flubendazole on non-small cell lung cancer A549 and H460 cell lines and the underlying mechanism.Methods CCK-8 assay was used to detect the effect of flubendazole at different concentrations on viability of both cell lines A549 and H460.We used western blot to detect the expression levels of autophagy-related proteins p62 and LC3 after flubendazole treatment.Cells were transfected with tandem fluorescent adenovirus(mRFP-GFP-LC3),and the impact of flubendazole treatment on autophagic flux were analyzed.Results Cell viability analysis showed a dose-dependent inhibitory effect on proliferation of both A549 and H460,comparing to cells without flubendazole treating(P<0.001).Level of p62 decreased and LC3 II/I ratio increased in cells treated with 2μmol/L flubendazole for 24 h and 48 h,compared to control groups(P<0.005).Red fluorescence signals increased in mRFP-GFP-LC3 transfected cells after flubendazole treating,suggesting an elevation in autophagic flux.Conclusion Flubendazole may inhibit the proliferation of A549 and H460 cells and promote autophagy.
作者
董庭君
路泽军
李婧姣
刘咏真
温居一
Tingjun DONG;Zejun LU;Jingjiao LI;Yongzhen LIU;Juyi WEN(The Second School of Clinical Medicine,Southern Medical University,Guangzhou 510280,China;Tumor Diagnosis and Treatment Center,The Sixth Medical Center of PLA General Hospital,Beijing 100048,China)
出处
《中国肺癌杂志》
CAS
CSCD
北大核心
2020年第5期306-313,共8页
Chinese Journal of Lung Cancer
关键词
氟苯达唑
肺肿瘤
自噬
Flubendazole
Lung neoplasms
Autophagy
