摘要
快速、准确鉴定出病原体是临床感染性疾病诊断和传染病预防控制的基础。高通量测序基因检测技术突破了传统检测手段的时效性、灵敏度等的局限,为病原体检测和研究提供了便捷、高效的途径。本综述以高通量测序技术发展过程为基础,回顾纳米孔三代测序技术,及其在病毒性传染病检测鉴定及研究中的应用,并对该技术的应用前景及可能存在的问题进行阐述,期望它能在病毒性传染病的防控方面发挥更大的作用。
Rapid identification of pathogens is the basis for clinical diagnosis and prevention and control of infectious disease.High throughput sequencing technology breaks through the limitation of traditional detection methods,such as timeliness and sensitivity,providing an efficient way for clinical detection and research.In this review,the development of high throughput sequencing technology was discussed,mainly by introducing the third-generation nanopore sequencing technology.At the same time,the application of nanopore sequencing technology about the detection and study in viral infectious diseases were reviewed,and the application prospects and possible problems were prospected.
作者
崔晓娴
李云逸
杨玉颖
张曦
李崇山
CUI Xiaoxian;LI Yunyi;YANG Yuying;ZHANG Xi;LI Chongshan(Division of Microbiology,Shanghai Municipal Center for Disease Control and Prevention,Shanghai 200336,China)
出处
《微生物与感染》
2020年第3期179-185,共7页
Journal of Microbes and Infections
基金
上海市卫生健康委员会科研课题(201940311)。
关键词
病毒性传染病
分子诊断技术
高通量测序
纳米孔三代测序技术
Viral infectious diseases
Molecular diagnostic
High throughput sequencing
Three generation sequencing technology of nanopore
