摘要
本试验以利多卡因为内标,采用反相高效液相色谱法同时检测人血浆中的布比卡因和罗哌卡因浓度。血浆样本经乙腈沉淀蛋白后进样分析。色谱柱为Zorbarx Eclipse XDB-C18柱,流动相为甲醇︰10 mmol/L磷酸盐缓冲液(43︰57),检测波长210 nm。结果显示,血浆中布比卡因和罗哌卡因分别在0.35~5.50μg/ml和0.21~3.34μg/ml内线性关系良好,定量限分别为0.35μg/ml和0.21μg/ml。本法的日间和日内精密度和准确度、长期稳定性及提取回收率均符合生物样本检测要求。本试验建立的方法灵敏、快速、准确,专属性强、重复性好,适用于血浆中布比卡因和罗哌卡因的血药浓度检测和药动学研究。
An HPLC method was established for the simultaneous determination of bupivacaine and ropivacaine in human plasma with lidocaine as the internal standard. The plasma samples were analyzed after precipitating protein with acetonitrile. The separation was performed on an Zorbarx Eclipse XDB-C18 column with the mobile phase of methanol and 10 mmol/L phosphate buffered solution(43︰57). The detection wavelength was 210 nm. It was linear for bupivacaine and ropivacaine in the range of 0.35-5.50 μg/ml and 0.21-3.34 μg/ml respectively with LLOQ of 0.35 μg/ml and 0.21 μg/ml. The intra-and inter-day precisions and accuracies, long-term stabilities and extraction recoveries in three quality control samples with different concentrations were all met the requirements of biological sample detection. This method was sensitive, rapid, accurate, reliable, and suitable for the determination of bupivacaine and ropivacaine in human plasma, which could provide references for pharmacokinetic study.
作者
石浩强
王曦晗
翟晓慧
DEVENDRA Ghimire
陈冰
薛庆生
SHI Haoqiang;WANG Xihan;ZHAI Xiaohui;DEVENDRA Ghimire;CHEN Bing(XUE Qingsheng2(1.Dept.of Pharmacy of Ruijin Hospital,Shanghai Jiaotong University,School of Medicine,Shanghai 200025;Dept.of Anesthesiology of Ruijin Hospital,Shanghai Jiaotong University,School of Medicine,Shanghai 200025)
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2020年第5期620-625,共6页
Chinese Journal of Pharmaceuticals
