摘要
目的建立工作场所空气中2-氨基吡啶、3-氨基吡啶和4-氨基吡啶的溶剂洗脱-气相色谱测定方法。方法采用硫酸溶液浸渍玻璃纤维滤纸采集工作场所空气中2-氨基吡啶、3-氨基吡啶和4-氨基吡啶,用氢氧化钠溶液洗脱后,毛细管色谱柱分离,氮磷检测器测定。结果本方法2-氨基吡啶、3-氨基吡啶和4-氨基吡啶的定量测定范围分别为0.30~800.00、0.80~800.00和2.00~800.00 mg/L,相关系数均大于0.9999;最低检出浓度分别为4.67、8.00和40.00μg/m^3,最低定量浓度分别为14.00、28.00、133.33μg/m^3(以采集45.00 L样品,洗脱液体积为3.00 mL计);平均洗脱效率分别为79.63%~98.61%、83.80%~101.42%和81.60%~100.29%;批内相对标准偏差(RSD)分别为1.13%~3.65%、1.47%~4.00%、1.94%~5.15%,批间RSD分别为1.77%~5.30%、2.06%~4.65%、2.59%~6.46%;样品在室温下可保存14 d以上。结论本方法适用于工作场所空气中2-氨基吡啶、3-氨基吡啶和4-氨基吡啶的检测。
Objective To establish a method for simultaneous detection of 2-aminopyridine,3-aminopyridine and 4-aminopyridine in workplace air by solvent eluting-gas chromatography.Methods 2-aminopyridine,3-Aminopyridine and 4-aminopyridine in workplace air were collected by glass fiber filter paper dipped with sulfuric acid solution,and eluted using sodium hydroxide solution.After that,the eluent was separated by capillary column,and finally detected using the nitrogen and phosphorus detector.Results The quantitative determination ranges of 2-aminopyridine,3-aminopyridine and 4-aminopyridine were 0.30-800.00,0.80-800.00 and 2.00-800.00 mg/L respectively,with the correlative coefficients greater than 0.9999.The minimum detectable concentrations were 4.67,8.00 and 40.00μg/m^3 respectively,and the minimum quantification concentrations were 14.00,28.00 and 133.33μg/m^3(45.00 L sample,3.00 mL eluent)respectively.The average elution efficiencies were 79.63%-98.61%,83.80%-101.42%and 81.60%-100.29%respectively.The within-run relative standard deviations(RSD)were 1.13%-3.65%,1.47%-4.00%and 1.94%-5.15%respectively,and the between-run RSD were 1.77%-5.30%,2.06%-4.65%and 2.59%-6.46%respectively.Samples were stable at room temperature for at least 14 days.Conclusion This method is appropriate to be applied for simultaneous detection of 2-aminopyridine,3-aminopyridine and 4-aminopyridine in workplace air.
作者
戎伟丰
凌伟洁
张耕
胡嘉雯
蒙瑞波
吴邦华
RONG Weifeng;LING Weijie;ZHANG Geng;HU Jiawen;MENG Ruibo;WU Banghua(Guangdong Province Hospital for Occupational Disease Prevention and Treatment,Guangdong Provincial Key Laboratory of Occupational Diseases Prevention and Treatment,Guangzhou,Guangdong 510300,China)
出处
《中国职业医学》
CAS
北大核心
2020年第1期86-90,共5页
China Occupational Medicine
基金
职业危害识别与控制湖北省重点实验室开放基金(OHIC2018G05)
广东省职业病防治重点实验室(2017B030314152)
广东省医学科研基金(A2019284)
