摘要
目的探讨血清特异miRNAs的检测在甲状腺乳头状癌诊断中的临床意义。方法 2017年1月~2019年5月收治的甲状腺乳头状癌病人92例为研究组,同期健康人群92例为对照组,同期甲状腺良性肿瘤病人92例为良性组,提取所有受试对象血清样本的总RNA,通过荧光定量PCR的方法检测miRNA-221、miRNA-222、miRNA-455的相对表达量,比较三组受试对象3种miRNA的表达情况,比较研究组不同临床病理特征病人3种miRNA的表达情况,并通过ROC工作曲线分析3种miRNA的诊断效能。结果研究组病人血清3种miRNA的表达均高于对照组;年龄超过45岁、肿瘤直径超过1 cm、单发病灶、存在腺外浸润的病人miRNA-455的表达升高;存在淋巴结转移和腺外浸润的病人miRNA-221和miRNA-222的表达显著升高,差异均有统计学意义(P<0.05)。miRNA-221、miRNA-222和miRNA-455表达在诊断甲状腺乳头状癌中的AUC分别为0.813、0.802和0.794。结论甲状腺乳头状癌病人血清miRNA-221、miRNA-222和miRNA-455的表达显著升高,可反映病人癌症病灶情况、转移情况以及浸润程度,血清特异性miRNA在诊断甲状腺乳头状癌中有一定的价值,其灵敏度和特异度仍需要进一步研究。
Objective To explore the clinical application of serum specific miRNAs in the diagnosis of thyroid papillary carcinoma.Methods Ninety-two patients with papillary thyroid cancer admitted to our hospital from January 2017 to May 2019 were selected as the study group.In the same period, 92 healthy people served as control group, while 92 benign thyroid tumors patients served as benign group.Total RNA was extracted from serum samples of all subjects,and the relative expression levels of microRNA-221, microRNA-222 and microRNA-455 were detected by fluorescence quantitative PCR.The expression of three kinds of microRNAs in three groups of subjects was compared.The expression of three kinds of microRNAs in patients with different clinicopathological characteristics in the study group was compared, and the diagnostic efficacy of the three kinds of microRNAs was analyzed by ROC working curve.Results The serum levels of three kinds of miRNAs in the study group were significantly higher than those in the control group;the expressions of miRNAs-455 were significantly higher in patients over 45 years old, with tumors larger than 1 cm, with single lesion and with extraglandular invasion;the expressions of miRNAs-221 and miRNAs-222 were significantly higher in patients with lymph node metastasis and extraglandular invasion.The above results were all P<0.05.The expression of miRNA-221, miRNA-222 and miRNA-455 in the diagnosis of papillary thyroid cancer were 0.813, 0.802 and 0.794, respectively.Conclusion The expression of serum miRNA-221, miRNA-222 and miRNA-455 in patients with thyroid papillary cancer is significantly increased. The expression of miRNAs can reflect the lesion, metastasis and infiltration of cancer. Serum-specific miRNAs has certain value in the diagnosis of thyroid papillary cancer, but the sensitivity and specificity of the diagnosis still need further study.
作者
林安忆
潘华锋
李琰
LIN Anyi;PAN Huafeng;LI Yan(Medical College,Wuhan University of Science and Technology,Wuhan 430007,China)
出处
《临床外科杂志》
2020年第3期231-234,共4页
Journal of Clinical Surgery
